General Information

    • DRAMP ID

    • DRAMP00863

    • Peptide Name

    • Kalata-B8 (Plant defensin)

    • Source

    • Oldenlandia affinis

    • Family

    • Belongs to the cyclotide family

    • Gene

    • Not found

    • Sequence

    • GSVLNCGETCLLGTCYTTGCTCNKYRVCTKD

    • Sequence Length

    • 31

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antiviral

    • Target Organism

      • [Ref.16207177]Virus: Human T-lymphoblast (CEM-SS) cells (EC50=2.5 µM).
      • [Ref.18008336]Virus:HIV:inhibition the cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells(EC50=2500 nM).

    • Hemolytic Activity

      • [Ref.20564013] It produced 7% hemolysis at the 62μM.

    • Cytotoxicity

      • [Ref.16207177] Kalata B8 inhibited the cytopathic effects of HIV-1 infection in cultured human T-lymphoblast (CEM-SS) cells with an antiviral cytoprotective concentration (EC50) of approx. 2.5 μM, while the cytotoxic concentration (IC50) was >11 μM.
      • [Ref.18008336]CEM-SS cells:IC50>11000 nM.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Cyclization (N termini to C termini)

    • C-terminal Modification

    • Cyclization (C termini to N termini)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys6 and Cys20; Cys10 and Cys22; Cys15 and Cys28.

    • Stereochemistry

    • L

    • Structure

    • Beta strand (3 strands; 7 residues)

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00863 helical wheel diagram

    • PDB ID

    • 2B38 resolved by NMR.

    • Predicted Structure

    • There is no predicted structure for DRAMP00863.

Physicochemical Information

    • Formula

    • C134H220N38O47S6

    • Absent Amino Acids

    • AFHIMPQW

    • Common Amino Acids

    • CT

    • Mass

    • 3307.81

    • PI

    • 7.76

    • Basic Residues

    • 3

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 5

    • Net Charge

    • +1

    • Boman Index

    • -39.65

    • Hydrophobicity

    • -0.023

    • Aliphatic Index

    • 56.45

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 3355

    • Absorbance 280nm

    • 111.83

    • Polar Residues

    • 21

DRAMP00863

DRAMP00863 chydropathy plot

Comments Information

    • Function

    • Probably participates in a plant defense mechanism. Inhibits the cytopathic effects of the human immunodeficiency virus. Has no hemolytic activity.

    • PTM

    • Kalata-B8 is a cyclic peptide which contains three disulfide bonds 6-20; 10-22; 15-28.

Literature Information

  • ·Literature 1

    • Title

    • Cyclotides as natural anti-HIV agents.

    • Reference

    • Biopolymers. 2008;90(1):51-60.

    • Author

    • Ireland DC, Wang CK, Wilson JA, Gustafson KR, Craik DJ.
  • ·Literature 2

    • Title

    • Kalata B8, a novel antiviral circular protein, exhibits conformational flexibility in the cystine knot motif.

    • Reference

    • Biochem J. 2006 Feb 1;393(Pt 3):619-626.

    • Author

    • Daly NL, Clark RJ, Plan MR, Craik DJ.
  • ·Literature 3

    • Title

    • The cyclotide fingerprint in Oldenlandia affinis: elucidation of chemically modified, linear and novel macrocyclic peptides.

    • Reference

    • Chembiochem. 2007 Jun 18;8(9):1001-1011.

    • Author

    • Plan MR, Göransson U, Clark RJ, Daly NL, Colgrave ML, Craik DJ.