General Information

    • DRAMP ID

    • DRAMP00868

    • Peptide Name

    • Kalata-B12 (Plant defensin)

    • Source

    • Oldenlandia affinis

    • Family

    • Belongs to the cyclotide family

    • Gene

    • Not found

    • Sequence

    • GSLCGDTCFVLGCNDSSCSCNYPICVKD

    • Sequence Length

    • 28

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Not found

    • Target Organism

    • No MICs found in DRAMP database

    • Hemolytic Activity

      • [Ref.21466163] It has almost negligible hemolytic activity.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • No specific N-terminal

    • C-terminal Modification

    • No specific C-terminal

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys4 and Cys18,Cys8 and Cys20,Cys13 and Cys25.

    • Stereochemistry

    • L

    • Structure

    • Beta strand (3 strands; 11 residues)

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00868 helical wheel diagram

    • PDB ID

    • 2KVX resolved by NMR.

    • Predicted Structure

    • There is no predicted structure for DRAMP00868.

Physicochemical Information

    • Formula

    • C117H183N31O43S6

    • Absent Amino Acids

    • AEHMQRW

    • Common Amino Acids

    • C

    • Mass

    • 2904.28

    • PI

    • 3.93

    • Basic Residues

    • 1

    • Acidic Residues

    • 3

    • Hydrophobic Residues

    • 6

    • Net Charge

    • -2

    • Boman Index

    • -24.98

    • Hydrophobicity

    • 0.318

    • Aliphatic Index

    • 62.5

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 1865

    • Absorbance 280nm

    • 69.07

    • Polar Residues

    • 17

DRAMP00868

DRAMP00868 chydropathy plot

Comments Information

    • Function

    • Probably participates in a plant defense mechanism.

    • PTM

    • Kalata-B12 are cyclic peptides which may contain three disulfide bonds 4-18; 8-20; 13-25.

Literature Information

  • ·Literature 1

    • Title

    • The cyclotide fingerprint in oldenlandia affinis: elucidation of chemically modified, linear and novel macrocyclic peptides.

    • Reference

    • Chembiochem. 2007 Jun 18;8(9):1001-1011.

    • Author

    • Plan MR, Göransson U, Clark RJ, Daly NL, Colgrave ML, Craik DJ.
  • ·Literature 2

    • Title

    • The role of conserved Glu residue on cyclotide stability and activity: a structural and functional study of kalata B12, a naturally occurring Glu to Asp mutant.

    • Reference

    • Biochemistry. 2011 May 17;50(19):4077-4086.

    • Author

    • Wang CK, Clark RJ, Harvey PJ, Rosengren KJ, Cemazar M, Craik DJ.