General Information
-
DRAMP ID
- DRAMP00876
-
Peptide Name
- Leaf cyclotide 2 (Vhl-2; Plant defensin)
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Source
- Viola hederacea (Australian violet)
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Family
- Belongs to the cyclotide family
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Gene
- Not found
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Sequence
- GLPVCGETCFTGTCYTNGCTCDPWPVCTRN
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Sequence Length
- 30
-
UniProt Entry
- P85231
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Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antiviral
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Target Organism
-
- [Ref.15824119]Virus: HIV(EC50= 0.87 μM)
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Hemolytic Activity
-
- [Ref:15824119]Has hemolytic activity
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Cytotoxicity
-
- Not included yet
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Binding Target
- cell membrabce
Structure Information
-
Linear/Cyclic
- Not included yet
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N-terminal Modification
- Not included yet
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C-terminal Modification
- Not included yet
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Nonterminal Modifications and Unusual Amino Acids
- Not included yet
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Stereochemistry
- Not included yet
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Structure
- Beta strand (5 strands; 6 residues)
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Structure Description
- Analysis of surface hydrophobicity and haemolytic activity for a range of cyclotides indicates a correlation between them, with increasing hydrophobicity resulting in increased haemolytic activity.
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Helical Wheel Diagram
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PDB ID
- 2KUK resolved by NMR.
- 2KUK-> 
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Predicted Structure
- There is no predicted structure for DRAMP00876.
Physicochemical Information
-
Formula
- C133H200N36O44S6
Absent Amino Acids
- AHIKMQS
Common Amino Acids
- CT
Mass
- 3199.63
PI
- 4.37
Basic Residues
- 1
Acidic Residues
- 2
Hydrophobic Residues
- 5
Net Charge
- -1
-
Boman Index
- -29.54
Hydrophobicity
- -0.043
Aliphatic Index
- 32.33
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 7365
Absorbance 280nm
- 253.97
Polar Residues
- 19
DRAMP00876
Comments Information
Function
- Probably participates in a plant defense mechanism. Has hemolytic activity.
Tissue specificity
- Expressed in leaves.
PTM
- This is a cyclic peptide which contains three disulfide bonds 5-19; 9-21; 14-27.
Literature Information
- ·Literature 1
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Title
- Isolation and characterization of novel cyclotides from Viola hederaceae: solution structure and anti-HIV activity of vhl-1, a leaf-specific expressed cyclotide.
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Pubmed ID
- 15824119
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Reference
- J Biol Chem. 2005 Jun 10;280(23):22395-22405.
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Author
- Chen B, Colgrave ML, Daly NL, Rosengren KJ, Gustafson KR, Craik DJ.
- ·Literature 2
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Title
- Structure and activity of the leaf-specific cyclotide vhl-2.
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Pubmed ID
- Pubmed ID is not available
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Reference
- Aust. J. Chem. 2010;63:771-778.
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Author
- Daly NL, Chen B, Nguyencong P, Craik DJ.