General Information

    • DRAMP ID

    • DRAMP00910

    • Peptide Name

    • Tricyclon-A (Cyclotides; Plant defensin)

    • Source

    • Viola arvensis (European field pansy) (Field violet)

    • Family

    • Belongs to the cyclotide family

    • Gene

    • Not found

    • Sequence

    • GGTIFDCGESCFLGTCYTKGCSCGEWKLCYGTN

    • Sequence Length

    • 33

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Not found

    • Target Organism

    • Cytolysis

    • Hemolytic Activity

      • [Ref:15893660] It has 2% hemolytic activity at 500 μM against human red blood cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • No specific N-terminal

    • C-terminal Modification

    • No specific C-terminal

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys7 and Cys21, Cys11 and Cys23, Cys16 and Cys29.

    • Stereochemistry

    • L

    • Structure

    • Beta strand (3 strands; 13 residues)

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00910 helical wheel diagram

    • PDB ID

    • 1YP8 resolved by NMR.

    • Predicted Structure

    • There is no predicted structure for DRAMP00910.

Physicochemical Information

    • Formula

    • C149H219N37O49S6

    • Absent Amino Acids

    • AHMPQRV

    • Common Amino Acids

    • G

    • Mass

    • 3504.96

    • PI

    • 4.68

    • Basic Residues

    • 2

    • Acidic Residues

    • 3

    • Hydrophobic Residues

    • 6

    • Net Charge

    • -1

    • Boman Index

    • -20.13

    • Hydrophobicity

    • 0.006

    • Aliphatic Index

    • 35.45

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 8855

    • Absorbance 280nm

    • 276.72

    • Polar Residues

    • 22

DRAMP00910

DRAMP00910 chydropathy plot

Comments Information

    • Function

    • Probably participates in a plant defense mechanism. Has a weak hemolytic activity.

    • PTM

    • Contains three disulfide bonds 7-21; 11-23; 16-29. Proteolytically processed in two steps to yield first two linear precursors, and then two cyclic peptides after removal of the N-terminal repeats (NTR) and short tails.

Literature Information

  • ·Literature 1

    • Title

    • Processing of a 22 kDa precursor protein to produce the circular protein tricyclon A.

    • Reference

    • Structure. 2005 May;13(5):691-701.

    • Author

    • Mulvenna JP, Sando L, Craik DJ.