General Information
-
DRAMP ID
- DRAMP00922
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Peptide Name
- Cyclotide vibi-J (Vbc3; Plant defensin)
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Source
- Viola biflora (Yellow wood violet)
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Family
- Belongs to the cyclotide family
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Gene
- Not found
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Sequence
- GTFPCGESCVWIPCISKVIGCACKSKVCYKN
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Sequence Length
- 31
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UniProt Entry
- B1NRR0
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Protein Existence
- Protein level
Activity Information
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Biological Activity
- Cytotoxic
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Target Organism
- No MICs found in DRAMP database
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Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
-
- Not included yet
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Binding Target
- Not found
Structure Information
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Linear/Cyclic
- Not included yet
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N-terminal Modification
- Not included yet
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C-terminal Modification
- Not included yet
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Nonterminal Modifications and Unusual Amino Acids
- Not included yet
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Stereochemistry
- Not included yet
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Structure
- Bridge
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Structure Description
- The cyclotides are head-to-tail macrocyclic and they have three disulfides arranged in a cystine knot, in which two disulfide bonds and their connecting backbone segments form an embedded ring that is penetrated by the third disulfide bond. Cyclotides exhibit a broad range of biological effects, including insecticidal, haemolytic, and cytotoxic effects.
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Helical Wheel Diagram
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PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP00922.
Physicochemical Information
-
Formula
- C145H231N37O40S6
Absent Amino Acids
- DHLMQR
Common Amino Acids
- C
Mass
- 3325.01
PI
- 8.65
Basic Residues
- 4
Acidic Residues
- 1
Hydrophobic Residues
- 9
Net Charge
- +3
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Boman Index
- -4.06
Hydrophobicity
- 0.432
Aliphatic Index
- 69.03
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 7365
Absorbance 280nm
- 245.5
Polar Residues
- 15
DRAMP00922
Comments Information
Function
- Probably participates in a plant defense mechanism and shows potent cytotoxic activity.
PTM
- Contains three disulfide bonds 5-21; 9-23; 14-28. This is a cyclic peptide (cross-link at G1-N31).
Literature Information
- ·Literature 1
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Title
- The alpine violet, Viola biflora, is a rich source of cyclotides with potent cytotoxicity.
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Pubmed ID
- 18191970
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Reference
- Phytochemistry. 2008 Feb;69(4):939-952.
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Author
- Herrmann A, Burman R, Mylne JS, Karlsson G, Gullbo J, Craik DJ, Clark RJ, Göransson U.