• DRAMP ID

    • DRAMP00926
    • Peptide Name

    • Violacin-A (Violacin-1; linearized uncyclotides; Plant defensin)
    • Source

    • Viola odorata (Sweet violet)
    • Family

    • Not found
    • Gene

    • Not found
    • Sequence

    • SAISCGETCFKFKCYTPRCSCSYPVCK
    • Sequence Length

    • 27
    • Protein Existence

    • Protein level
    • Biological Activity

    • Antimicrobial
    • Target Organism

    • [Ref.16488428]show insecticidal, anti-HIV, antimicrobial, antineurotensive, cytotoxic and haemolytic activity
    • Hemolytic Activity

      • [Ref:16488428]10% hemolytic activity at 1.0μM and 35% hemolytic activity at 1.5μM against human type A red blood cells
    • Cytotoxicity

      • Not included yet
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Not included yet
    • N-terminal Modification

    • Not included yet
    • C-terminal Modification

    • Not included yet
    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet
    • Stereochemistry

    • Not included yet
    • Structure

    • Beta strand (4 strands; 9 residues)
    • Structure Description

    • The three-dimensional solution structure of violacin A is determined and found to adopt the cystine knot fold of native cyclotides. (Ref.2)
    • Helical Wheel Diagram

    • DRAMP00926 helical wheel diagram
    • PDB ID

    • 2FQA resolved by NMR.
  • 2FQA-> 
    • Predicted Structure

    • There is no predicted structure for DRAMP00926.
    • Formula

    • C129H199N33O38S6
    • Absent Amino Acids

    • DHLMNQW
    • Common Amino Acids

    • C
    • Mass

    • 3012.56
    • PI

    • 8.64
    • Basic Residues

    • 4
    • Acidic Residues

    • 1
    • Hydrophobic Residues

    • 5
    • Net Charge

    • +3
    • Boman Index

    • -32.05
    • Hydrophobicity

    • 0.022
    • Aliphatic Index

    • 28.89
    • Half Life

      • Mammalian:1.9 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 3355
    • Absorbance 280nm

    • 129.04
    • Polar Residues

    • 15

DRAMP00926

DRAMP00926 chydropathy plot
    • Function

    • Probably participates in a plant defense mechanism. Has low hemolytic activity.
    • Miscellaneous

    • The presence of a premature stop codon inhibits the translation of a key Asn residue that is thought to be required for cyclization.
    • PTM

    • Contains three disulfide bonds 5-19; 9-21; 14-26.
  • ·Literature 1
    • Title

    • A novel suite of cyclotides from Viola odorata: sequence variation and the implications for structure, function and stability..
    • Reference

    • Biochem J. 2006 Nov 15;400(1):1-12.
    • Author

    • Ireland DC, Colgrave ML, Craik DJ.
  • ·Literature 2
    • Title

    • Discovery and characterization of a linear cyclotide from Viola odorata: implications for the processing of circular proteins.
    • Reference

    • J Mol Biol. 2006 Apr 14;357(5):1522-1535.
    • Author

    • Ireland DC, Colgrave ML, Nguyencong P, Daly NL, Craik DJ.