General Information

    • DRAMP ID

    • DRAMP00935

    • Peptide Name

    • Ribosome-inactivating protein trichokirin (rRNA N-glycosidase; Plant defensin)

    • Source

    • Trichosanthes kirilowii (Chinese snake gourd) (Chinese cucumber)

    • Family

    • Belongs to the ribosome-inactivating protein family (Type 1 RIP subfamily)

    • Gene

    • Not found

    • Sequence

    • DVSFSLSGGGTASYEK

    • Sequence Length

    • 16

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Cytotoxic

    • Target Organism

    • No MICs found in DRAMP database

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00935 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00935.

Physicochemical Information

    • Formula

    • C69H105N17O27

    • Absent Amino Acids

    • CHIMNPQRW

    • Common Amino Acids

    • S

    • Mass

    • 1604.69

    • PI

    • 4.37

    • Basic Residues

    • 1

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 4

    • Net Charge

    • -1

    • Boman Index

    • -20.82

    • Hydrophobicity

    • -0.294

    • Aliphatic Index

    • 48.75

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 1490

    • Absorbance 280nm

    • 99.33

    • Polar Residues

    • 9

DRAMP00935

Comments Information

    • Catalytic activity

    • Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

    • PTM

    • Glycosylated.

Literature Information

  • ·Literature 1

    • Title

    • Trichokirin, a ribosome-inactivating protein from the seeds of Trichosanthes kirilowii Maximowicz. Purification, partial characterization and use for preparation of immunotoxins.

    • Reference

    • Eur J Biochem. 1988 Oct 1;176(3):581-588.

    • Author

    • Casellas P, Dussossoy D, Falasca AI, Barbieri L, Guillemot JC, Ferrara P, Bolognesi A, Cenini P, Stirpe F.