General Information

    • DRAMP ID

    • DRAMP00940

    • Peptide Name

    • Crambin (Plant defensin)

    • Source

    • Crambe hispanica subsp. abyssinica (Abyssinian kale) (Crambe abyssinica)

    • Family

    • Belongs to the thionin family

    • Gene

    • THI2

    • Sequence

    • TTCCPSIVARSNFNVCRLPGTPEALCATYTGCIIIPGATCPGDYAN

    • Sequence Length

    • 46

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Cytotoxic

    • Target Organism

    • No MICs found in DRAMP database

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Combine helix and strand structure

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00940 helical wheel diagram

    • PDB ID

    • 3NIR resolved by X-ray.

    • Predicted Structure

    • There is no predicted structure for DRAMP00940.

Physicochemical Information

    • Formula

    • C202H321N55O64S6

    • Absent Amino Acids

    • HKMQW

    • Common Amino Acids

    • CT

    • Mass

    • 4736.46

    • PI

    • 5.73

    • Basic Residues

    • 2

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 14

    • Net Charge

    • 0

    • Boman Index

    • -26.72

    • Hydrophobicity

    • 0.354

    • Aliphatic Index

    • 74.35

    • Half Life

      • Mammalian:7.2 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 3355

    • Absorbance 280nm

    • 74.56

    • Polar Residues

    • 23

DRAMP00940

DRAMP00940 chydropathy plot

Comments Information

    • Function

    • The Function

    • Miscellaneous

    • Two isoforms exists, a major form PL (shown here) and a minor form SI.

    • PTM

    • Contains three disulfide bonds 3-40; 4-32; 16-26.

Literature Information

  • ·Literature 1

    • Title

    • Primary structure of the hydrophobic plant protein crambin.

    • Reference

    • Biochemistry. 1981 Sep 15;20(19):5437-5443.

    • Author

    • Teeter MM, Mazer JA, L'Italien JJ.
  • ·Literature 2

    • Title

    • Crystal structure of small protein crambin at 0.48Å resolution.

    • Reference

    • Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Apr 1;67(Pt 4):424-428.

    • Author

    • Schmidt A, Teeter M, Weckert E, Lamzin VS.