General Information
-
DRAMP ID
- DRAMP00977
-
Peptide Name
- Antimicrobial peptide 2 (AC-AMP2; Plant defensin)
-
Source
- Amaranthus caudatus (Love-lies-bleeding) (Inca-wheat)
-
Family
- Not found
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Gene
- Not found
-
Sequence
- VGECVRGRCPSGMCCSQFGYCGKGPKYCGR
-
Sequence Length
- 30
-
UniProt Entry
- P27275
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Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Antifungal
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Target Organism
-
- Gram-positive bacteria: Bacillus megaterium (IC50=40 µg/ml), Sarcina lutea (IC50=250 µg/ml).
- Fungi: Alternaria brassicola (IC50=7 µg/ml), Ascochyta pisi (IC50=8 µg/ml), Botrytis cinera (IC50=10 µg/ml), Colletotrichum lindemuthianum (IC50=8 µg/ml), Fusarium culmorum (IC50=2 µg/ml), Trichoderma hamatum (IC50=7 µg/ml), Verticillium dahliae (IC50=6 µg/ml).
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Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
-
- Not included yet
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Binding Target
- Chitin-binding
Structure Information
-
Linear/Cyclic
- Not included yet
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N-terminal Modification
- Not included yet
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C-terminal Modification
- Not included yet
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Nonterminal Modifications and Unusual Amino Acids
- Not included yet
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Stereochemistry
- Not included yet
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Structure
- Beta strand
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Structure Description
- The main structural element is an antiparallel beta-sheet from Met13 to Lys23 including a betaI-turn over Gln17-Phel8 with a beta bulge at Gly19. In addition, a beta'I turn over Arg6-Gly7, a beta'III turn over Ser11-Gly12 and a helical turn from Gly24 to Cys28 are identified.
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Helical Wheel Diagram
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Predicted Structure
- There is no predicted structure for DRAMP00977.
Physicochemical Information
-
Formula
- C130H206N42O38S7
Absent Amino Acids
- ADHILNTW
Common Amino Acids
- G
Mass
- 3189.74
PI
- 8.92
Basic Residues
- 5
Acidic Residues
- 1
Hydrophobic Residues
- 3
Net Charge
- +4
-
Boman Index
- -47.62
Hydrophobicity
- -0.347
Aliphatic Index
- 19.33
Half Life
-
- Mammalian:100 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 3355
Absorbance 280nm
- 115.69
Polar Residues
- 17
DRAMP00977
![DRAMP00977 chydropathy plot](http://dramp.cpu-bioinfor.org/tmp/all_info_png/pepwindow_DRAMP00977.1.png)
Comments Information
Function
- Chitin-binding protein with a defensive Function
Miscellaneous
- Its chitin-binding activity is strongly inhibited by divalent cations.
PTM
- Contains three disulfide bonds 4-15; 9-21; 14-28.
Literature Information
- ·Literature 1
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Title
- Antimicrobial peptides from Amaranthus caudatus seeds with sequence homology to the cysteine/glycine-rich domain of chitin-binding proteins.
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Pubmed ID
- 1567877
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Reference
- Biochemistry. 1992 May 5;31(17):4308-4314.
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Author
- Broekaert W.F, Marien W, Terras F.R.G, de Bolle M.F.C, Proost P, van Damme J, Dillen L, Claeys M, Rees S.B, Vanderleyden J, Cammue B.P.A.
- ·Literature 2
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Title
- H NMR study of the solution structure of Ac-AMP2, a sugar binding antimicrobial protein isolated from Amaranthus caudatus.
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Pubmed ID
- 8627629
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Reference
- J Mol Biol. 1996 May 3;258(2):322-333.
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Author
- Martins JC, Maes D, Loris R, Pepermans HA, Wyns L, Willem R, Verheyden P.
- ·Literature 3
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Title
-
Pubmed ID
- 9176817
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Reference
- J Pept Res. 1997 Apr;49(4):336-340.
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Author
- el Bouyoussfi M, Laus G, Verhey