• DRAMP ID

    • DRAMP00979
    • Peptide Name

    • Antifungal peptide 2 (EAFP2; Plant defensin)
    • Source

    • Eucommia ulmoides (Hardy rubber tree)
    • Family

    • Belongs to the hevein-like family
    • Gene

    • N/A
    • Sequence

    • QTCASRCPRPCNAGLCCSIYGYCGSGAAYCGAGNCRCQCRG
    • Sequence Length

    • 41
    • Evidence code

    • Protein level
    • Biological Activity

    • Antifungal, Antimicrobial
    • Target Organism

      • Fungi: Phytophthora infestans, Ascochyta lycopersici (IC50=109 µg/ml), Verticillium dahliae, Gibberella zeae, Alternaria nicotianae, Fusarium moniliforme (IC50=18 µg/ml), Fusarium oxysporum (IC50=94 µg/ml) and Colletotrichum gossypii (IC50=56 µg/ml).
    • Hemolytic Activity

      • N/A
    • Cytotoxicity

      • Not included yet
    • Binding Target

    • Chitin-binding
    • Linear/Cyclic

    • Not included yet
    • N-terminal Modification

    • Not included yet
    • C-terminal Modification

    • Not included yet
    • Other Modifications and Unusual Amino Acids

    • Not included yet
    • Stereochemistry

    • Not included yet
    • Structure

    • Combine helix and strand structure
    • Structure Description

    • EAFP2 adopts a compact global fold composed of a 3(10) helix (Cys3-Arg6), an alpha-helix (Ala27-Cys31) and a three-stranded antiparallel beta-sheet (Cys16-Ser18, Cys23-Ser25, and Cys35-Cys37) and cross-linked by five disulfide bridges. The tertiary structure of EAFP2 shows a chitin-binding domain.
    • PDB ID

    • 1P9G resolved by X-ray.
    • DRAMP00979 helical wheel diagram
    • 1P9G-> 
    • Formula

    • C163H259N57O53S10
    • Absent Amino Acids

    • DEFHKMVW
    • Common Amino Acids

    • C
    • Mass

    • 4899.49
    • PI

    • 8.26
    • Basic Residues

    • 4
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 7
    • Boman Index

    • -58.96
    • Hydrophobicity

    • -6.59
    • Aliphatic Index

    • 31.22
    • Half Life

      • Mammalian:0.8 hour
      • Yeast:10 min
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 5095
    • Absorbance 280nm

    • 127.38
    • Polar Residues

    • 26

DRAMP00979

DRAMP00979 chydropathy plot
    • Function

    • Has antifungal activity.
    • PTM

    • Contains five disulfide bonds 3-17; 7-37; 11-23; 16-30; 35-39, and Pyrrolidone carboxylic acid at position 1.
  • Literature 1
    • Title

    • Two novel antifungal peptides distinct with a five-disulfide motif from the bark of Eucommia ulmoides Oliv.
    • Reference

    • FEBS Lett. 2002 Jun 19;521(1-3):87-90.
    • Author

    • Huang R.-H, Xiang Y, Liu X.-Z, Zhang Y, Hu Z, Wang D.-C.
  • Literature 2
    • Title

    • Crystal structure of a novel antifungal protein distinct with five disulfide bridges from Eucommia ulmoides Oliver at an atomic resolution.
    • Reference

    • J Struct Biol. 2004 Oct;148(1):86-97.
    • Author

    • Xiang Y, Huang RH, Liu XZ, Zhang Y, Wang DC.
  • Literature 3
    • Title

    • Solution structure of Eucommia antifungal peptide: a novel structural model distinct with a five-disulfide motif.
    • Reference

    • Biochemistry. 2004 May 25;43(20):6005-6012.
    • Author

    • Huang RH, Xiang Y, Tu GZ, Zhang Y, Wang DC.