• • DRAMP ID

  • DRAMP00979

• • Peptide Name

  • Antifungal peptide 2 (EAFP2; Plant defensin)

• • Source

  • Eucommia ulmoides (Hardy rubber tree)

• • Family

  • Belongs to the hevein-like family

• • Gene

  • Not found

• • Sequence

  • QTCASRCPRPCNAGLCCSIYGYCGSGAAYCGAGNCRCQCRG

• • Sequence Length

  • 41

• • UniProt Entry

  • P83597

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antifungal

• • Target Organism

  • • Fungi: Phytophthora infestans, Ascochyta lycopersici (IC50=109 µg/ml), Verticillium dahliae, Gibberella zeae, Alternaria nicotianae, Fusarium moniliforme (IC50=18 µg/ml), Fusarium oxysporum (IC50=94 µg/ml) and Colletotrichum gossypii (IC50=56 µg/ml).

• • Hemolytic Activity

  • • No hemolysis information or data found in the reference(s) presented in this entry

• • Cytotoxicity

  • • Not included yet

• • Binding Target

  • Chitin-binding

• • Linear/Cyclic

  • Not included yet

• • N-terminal Modification

  • Not included yet

• • C-terminal Modification

  • Not included yet

• • Nonterminal Modifications and Unusual Amino Acids

  • Not included yet

• • Stereochemistry

  • Not included yet

• • Structure

  • Combine helix and strand structure

• • Structure Description

  • EAFP2 adopts a compact global fold composed of a 3(10) helix (Cys3-Arg6), an alpha-helix (Ala27-Cys31) and a three-stranded antiparallel beta-sheet (Cys16-Ser18, Cys23-Ser25, and Cys35-Cys37) and cross-linked by five disulfide bridges. The tertiary structure of EAFP2 shows a chitin-binding domain.

• • Helical Wheel Diagram

  • 

• • PDB ID

  • 1P9G resolved by X-ray.
• 1P9G-> 

• Predicted Structure

• There is no predicted structure for DRAMP00979.

DRAMP00979

• • Function

  • Has antifungal activity.

• • PTM

  • Contains five disulfide bonds 3-17; 7-37; 11-23; 16-30; 35-39, and Pyrrolidone carboxylic acid at position 1.

• ·Literature 1

• • Title

  • Two novel antifungal peptides distinct with a five-disulfide motif from the bark of Eucommia ulmoides Oliv.

• • Pubmed ID

  • 12067732

• • Reference

  • FEBS Lett. 2002 Jun 19;521(1-3):87-90.

• • Author

  • Huang R.-H, Xiang Y, Liu X.-Z, Zhang Y, Hu Z, Wang D.-C.

• ·Literature 2

• • Title

  • Crystal structure of a novel antifungal protein distinct with five disulfide bridges from Eucommia ulmoides Oliver at an atomic resolution.

• • Pubmed ID

  • 15363789

• • Reference

  • J Struct Biol. 2004 Oct;148(1):86-97.

• • Author

  • Xiang Y, Huang RH, Liu XZ, Zhang Y, Wang DC.

• ·Literature 3

• • Title

  • Solution structure of Eucommia antifungal peptide: a novel structural model distinct with a five-disulfide motif.

• • Pubmed ID

  • 15147184

• • Reference

  • Biochemistry. 2004 May 25;43(20):6005-6012.

• • Author

  • Huang RH, Xiang Y, Tu GZ, Zhang Y, Wang DC.