General Information

    • DRAMP ID

    • DRAMP00994

    • Peptide Name

    • IB-AMP1 (IBAMP1; Basic peptide AMP1; Plants)

    • Source

    • Impatiens balsamina (Balsam)

    • Family

    • Not found

    • Gene

    • AMP

    • Sequence

    • QWGRRCCGWGPGRRYCVRWC

    • Sequence Length

    • 20

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Antifungal

    • Target Organism

      • Gram-positive bacteria: Bacillus subtilis (IC50=10 µg/ml), Micrococcus luteus (IC50=10 µg/ml), Staphylococcus aureus (IC50=30 µg/ml), Streptococcus faecalis (IC50=6 µg/ml).
      • Fungi: Alternaria longipes (IC50=3 µg/ml), Botrytis cinerea (IC50=12 µg/ml), Cladosporium sphaerospermum (IC50=1 µg/ml), Fusarium culmorum (IC50=1 µg/ml), Penicillium digitatum (IC50=3 µg/ml), Trichoderma viride (IC50=6 µg/ml), Verticillium alboatrum (IC50=3 µg/ml).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Bridge

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00994 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00994.

Physicochemical Information

    • Formula

    • C107H157N39O23S4

    • Absent Amino Acids

    • ADEFHIKLMNST

    • Common Amino Acids

    • R

    • Mass

    • 2485.91

    • PI

    • 9.88

    • Basic Residues

    • 5

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 4

    • Net Charge

    • +5

    • Boman Index

    • -60.37

    • Hydrophobicity

    • -0.95

    • Aliphatic Index

    • 14.5

    • Half Life

      • Mammalian:0.8 hour
      • Yeast:10 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 18240

    • Absorbance 280nm

    • 960

    • Polar Residues

    • 9

DRAMP00994

DRAMP00994 chydropathy plot

Comments Information

    • Developmental stage

    • Highly expressed in dry mature seed and by the stages 2-5 of developing seed. The peptide IB-AMP1 is also detected at early stages of germination (24 hours and 48 hours postgermination).

    • PTM

    • Contains two disulfide bonds C6-C16; C7-C2

Literature Information

  • ·Literature 1

    • Title

    • A novel family of small cysteine-rich antimicrobial peptides from seed of Impatiens balsamina is derived from a single precursor protein.

    • Reference

    • J Biol Chem. 1997 Sep 26;272(39):24480-24487.

    • Author

    • Tailor RH, Acland DP, Attenborough S, Cammue BP, Evans IJ, Osborn RW, Ray JA, Rees SB, Broekaert WF.
  • ·Literature 2

    • Title

    • Structural studies of Impatiens balsamina antimicrobial protein (Ib-AMP1).

    • Reference

    • Biochemistry. 1998 Jan 27;37(4):983-990.

    • Author

    • Patel SU, Osborn R, Rees S, Thornton JM.