General Information
-
DRAMP ID
- DRAMP00997
-
Peptide Name
- IB-AMP4 (IBAMP4; Basic peptide AMP4; Plants)
-
Source
- Impatiens balsamina (Balsam)
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- QWGRRCCGWGPGRRYCRRWC
-
Sequence Length
- 20
-
UniProt Entry
- O24006
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
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Target Organism
-
- Gram-positive bacteria: Bacillus subtilis (IC50=5 µg/ml), Micrococcus luteus (IC50=5 µg/ml), Staphylococcus aureus (IC50=20 µg/ml), Streptococcus faecalis (IC50=5 µg/ml);
- Gram-negative bacteria: Xanthomonas campestris (IC50=6 µg/ml), Xanthomonas oryzae (IC50=15 µg/ml).
- Fungi: Alternaria longipes (IC50=3 µg/ml), Botrytis cinerea (IC50=6 µg/ml), Cladosporium sphaerospermum (IC50=1 µg/ml), Fusarium culmorum (IC50=1 µg/ml), Penicillium digitatum (IC50=3 µg/ml), Trichoderma viride (IC50=6 µg/ml), Verticillium alboatrum (IC50=6 µg/ml).
-
Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
-
- Not included yet
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Not included yet
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N-terminal Modification
- Not included yet
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C-terminal Modification
- Not included yet
-
Nonterminal Modifications and Unusual Amino Acids
- Not included yet
-
Stereochemistry
- Not included yet
-
Structure
- Bridge
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP00997.
Physicochemical Information
-
Formula
- C108H160N42O23S4
Absent Amino Acids
- ADEFHIKLMNSTV
Common Amino Acids
- R
Mass
- 2542.97
PI
- 10.66
Basic Residues
- 6
Acidic Residues
- 0
Hydrophobic Residues
- 3
Net Charge
- +6
-
Boman Index
- -79.33
Hydrophobicity
- -1.385
Aliphatic Index
- 0
Half Life
-
- Mammalian:0.8 hour
- Yeast:10 min
- E.coli:>10 hour
Extinction Coefficient Cystines
- 18240
Absorbance 280nm
- 960
Polar Residues
- 9
DRAMP00997
Comments Information
Developmental stage
- Highly expressed in dry mature seed and by the stages 2-5 of developing seed. PTM
Literature Information
- ·Literature 1
-
Title
- A novel family of small cysteine-rich antimicrobial peptides from seed of Impatiens balsamina is derived from a single precursor protein.
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Pubmed ID
- 9305910
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Reference
- J Biol Chem. 1997 Sep 26;272(39):24480-24487.
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Author
- Tailor RH, Acland DP, Attenborough S, Cammue BP, Evans IJ, Osborn RW, Ray JA, Rees SB, Broekaert WF.
- ·Literature 2
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Title
- Structural studies of Impatiens balsamina antimicrobial protein (Ib-AMP1).
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Pubmed ID
- 9454588
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Reference
- Biochemistry. 1998 Jan 27;37(4):983-990.
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Author
- Patel SU, Osborn R, Rees S, Thornton JM.