General Information
-
DRAMP ID
- DRAMP01025
-
Peptide Name
- Vicilin-like Antimicrobial peptide 2a (MiAMP2a; Plant defensin)
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Source
- Macadamia integrifolia (Macadamia nut)
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Family
- Belongs to the vicilin-like family
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Gene
- AMP2-2
-
Sequence
- ESEFDRQEYEECKRQCMQLETSGQMRRCVSQCDKRFEEDIDWSKYDNQD
-
Sequence Length
- 49
-
UniProt Entry
- Q9SPL4
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Protein Existence
- Transcript level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Antifungal
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Target Organism
-
- Gram-positive bacteria: (- Ca2+, + Ca2+): Clavibacter michiganensis (IC50=50, >50 µg/ml).
- Fungi (- Ca2+, + Ca2+): Alternaria helianthi (IC50=5-10, ND µg/ml), Ceratocystis paradoxa (IC50=20-50, >50 µg/ml), Cercospora nicotianae (IC50=5-10, 5-10 µg/ml), Chalara elegans (IC50=2-5, 10-20 µg/ml), Fusarium oxysporum (IC50=10, 20-50 µg/ml), Leptosphaeria maculans (IC50=25, >50 µg/ml), Sclerotinia sclerotiorum (IC50=20-50, >50 µg/ml), Verticillium dahliae (IC50=5-10, >50 µg/ml), Saccharomyces cerevisiae (IC50=20-50, >50 µg/ml), Phytophthora cryptogea (IC50=5-10, 10-25 µg/ml), Phytophthora parasitica nicotianae (IC50=10-20, >50 µg/ml).
- [NOTE:, Ca2+ = Medium with low content of CaCl2 (50 µM); + Ca2+ = Medium supplemented with high concentration of CaCl2 (1 mM)]
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Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
-
- Not included yet
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Not included yet
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N-terminal Modification
- Not included yet
-
C-terminal Modification
- Not included yet
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Nonterminal Modifications and Unusual Amino Acids
- Not included yet
-
Stereochemistry
- Not included yet
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Structure
- Not found
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP01025.
Physicochemical Information
-
Formula
- C250H383N75O92S6
Absent Amino Acids
- AHP
Common Amino Acids
- E
Mass
- 6103.6
PI
- 4.43
Basic Residues
- 8
Acidic Residues
- 14
Hydrophobic Residues
- 6
Net Charge
- -6
-
Boman Index
- -221.45
Hydrophobicity
- -1.706
Aliphatic Index
- 21.84
Half Life
-
- Mammalian:1 hour
- Yeast:30 min
- E.coli:>10 hour
Extinction Coefficient Cystines
- 8730
Absorbance 280nm
- 181.88
Polar Residues
- 13
DRAMP01025
Comments Information
Comment
- No comments found on DRAMP database
Literature Information
- ·Literature 1
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Title
- A family of antimicrobial peptides is produced by processing of a 7S globulin protein in Macadamia integrifolia kernels.
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Pubmed ID
- 10571855
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Reference
- Plant J. 1999 Sep;19(6):699-710.
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Author
- Marcus JP, Green JL, Goulter KC, Manners JM.