General Information

    • DRAMP ID

    • DRAMP01046

    • Peptide Name

    • Shepherin I (fragement of shep-GRP; Plants)

    • Source

    • Capsella bursa-pastoris (shepherd's purse)

    • Family

    • Not found

    • Gene

    • GRP

    • Sequence

    • GYGGHGGHGGHGGHGGHGGHGHGGGGHG

    • Sequence Length

    • 28

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-negative bacteria: Erwinia herbicola (IC50=62 µg/ml), Escherichia coli (IC50<2.5 µg/ml), Pseudomonas putida (IC50<2.5 µg/ml), Pseudomonas syringae (IC50<2.5 µg/ml), Salmonella typhimurium (IC50<2.5 µg/ml), Serratia sp. (IC50=8 µg/ml).
      • Fungi: Candida albicans (IC50=8 µg/ml), Cryptococcus neoformans (IC50<2.5 µg/ml), Saccharomyces cerevisiae (IC50<7 µg/ml), Alternaria alternata (IC50<7 µg/ml), Aspergillus flavus (IC50=65 µg/ml), Fusarium culmorum (IC50=72 µg/ml).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Circular dichroism (CD) spectra of shepherin I showes that shepherin I in 50% trifluoroethanol has 66.7% random coils, without any alpha-helices.

    • Helical Wheel Diagram

    • DRAMP01046 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP01046.

Physicochemical Information

    • Formula

    • C95H124N44O30

    • Absent Amino Acids

    • ACDEFIKLMNPQRSTVW

    • Common Amino Acids

    • G

    • Mass

    • 2362.31

    • PI

    • 7.28

    • Basic Residues

    • 8

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 0

    • Net Charge

    • +8

    • Boman Index

    • -19.56

    • Hydrophobicity

    • -1.232

    • Aliphatic Index

    • 0

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 1490

    • Absorbance 280nm

    • 55.19

    • Polar Residues

    • 20

DRAMP01046

DRAMP01046 chydropathy plot

Comments Information

    • Function

    • These antimicrobial peptides exhibit antimicrobial activity against Gram-negative bacteria and fungi.

    • Tissue specificity

    • Expressed in roots but not in leaves and stems.

Literature Information

  • ·Literature 1

    • Title

    • Characterization and cDNA cloning of two glycine- and histidine-rich antimicrobial peptides from the roots of shepherd's purse, Capsella bursa-pastoris.

    • Reference

    • Plant Mol Biol. 2000 Sep;44(2):187-197.

    • Author

    • Park CJ, Park CB, Hong SS, Lee HS, Lee SY, Kim SC.