• DRAMP ID

    • DRAMP01064
    • Peptide Name

    • Anticancerous peptide 1 (Cr-ACP1; Plants)
    • Source

    • Cycas revoluta (Sago palm)
    • Family

    • Unknown
    • Gene

    • Unknown
    • Sequence

    • AWKLFDDGV
    • Sequence Length

    • 9
    • Evidence code

    • Protein level
    • Biological Activity

    • Anticancer, Antibacterial, Anti-Gram+, Anti-Gram-, Antimicrobial
    • Target Organism

      • [Ref.21882228]Gram-positive bacteria: Staphylococcus epidermidis (MIC=60 µM), Bacillus subtilis (MIC=30 µM);
      • Gram-negative bacteria: Pseudomonas aeruginosa (MIC=30 µM), EEscherichia coli strain ATCC 8739 (MIC=30 µM).
    • Hemolytic activity

      • [Ref:21882228]8% hemolytic activity at 1 mM against 6-week-old male BALB/c mice red blood cells
    • Binding Target

    • DNA
    • Structure

    • Unknown
    • Structure Description

    • Unknown
    • DRAMP01064 helical wheel diagram
    • Formula

    • C50H71N11O14
    • Absent Amino Acids

    • CEHIMNPQRSTY
    • Common Amino Acids

    • D
    • Mass

    • 1193.07
    • PI

    • 4.11
    • Basic Residues

    • 1
    • Acidic Residues

    • 2
    • Hydrophobic Residues

    • 5
    • Boman Index

    • -5.97
    • Hydrophobicity

    • 4.44
    • Aliphatic Index

    • 86.67
    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 5500
    • Absorbance 280nm

    • 687.5
    • Polar Residues

    • 1

DRAMP01064

    • Function

    • The synthetic peptide inhibits cell proliferation and induces apoptosis in cancer-derived cell lines Hep2 (IC50=1.5 mM) and HCT15 and, to a lesser extent, in non-cancerous NIH/3T3 cells. The mode of action is presumably an arrest in the G0/G1 phase. Antiproliferative, proapoptotic and DNA-binding activities are increased by acetylation at Ala-1 and Lys-3. Has a weak hemolytic activity against mouse erythrocytes. Has antibacterial activity. Antibacterial activity is decreased by acetylation.
  • Literature 1
    • Reference

    • J Cell Biochem. 2012 Jan;113(1):184-193.
    • Author

    • Mandal SM, Migliolo L, Das S, Mandal M, Franco OL, Hazra TK.
    • Title

    • Identification and characterization of a bactericidal and proapoptotic peptide from Cycas revoluta seeds with DNA binding properties.