General Information

    • DRAMP ID

    • DRAMP01082

    • Peptide Name

    • Alyteserin-2a (toads, amphibians, animals)

    • Source

    • Alytes obstetricans (European midwife toad)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • ILGKLLSTAAGLLSNL

    • Sequence Length

    • 16

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Cytotoxicity

    • Target Organism

      • [Ref.19463738]Gram-positive bacterium: Staphylococcus aureus (MIC=50 µM).

    • Hemolytic Activity

      • [Ref:19463738] LC50 = 135 μM against human erthrocytes.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • α-Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L (to be determined)

    • Structure

    • Alpha helix

    • Structure Description

    • It is helical in 50% TFE.

    • Helical Wheel Diagram

    • DRAMP01082 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP01082.

Physicochemical Information

    • Formula

    • C72H130N18O21

    • Absent Amino Acids

    • CDEFHMPQRVWY

    • Common Amino Acids

    • L

    • Mass

    • 1583.93

    • PI

    • 8.75

    • Basic Residues

    • 1

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +1

    • Boman Index

    • 18.38

    • Hydrophobicity

    • 1.275

    • Aliphatic Index

    • 183.13

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 6

DRAMP01082

Comments Information

    • Function

    • The alyteserin-2a is more potent against the Gram-positive bacteria Staphylococcus aureus. The hemolytic activity against human erythrocytes of all peptides tested is relatively weak.

    • Structural similarity

    • Alyteserin-2a and alyteserin-2b and -2c show limited sequence identity with bombinin H6, present in the skins of frogs of the family Bombinatoridae.

Literature Information

  • ·Literature 1

    • Title

    • The alyteserins: two families of antimicrobial peptides from the skin secretions of the midwife toad Alytes obstetricans (Alytidae).

    • Reference

    • Peptides. 2009 Jun;30(6):1069-1073.

    • Author

    • Conlon JM, Demandt A, Nielsen PF, Leprince J, Vaudry H, Woodhams DC.