• DRAMP ID

    • DRAMP01085
    • Peptide Name

    • Alyteserin-1c (toads, amphibians, animals)
    • Source

    • Alytes obstetricans (Common midwife toad) (Bufo obstetricans)
    • Family

    • Unknown
    • Gene

    • Unknown
    • Sequence

    • GLKEIFKAGLGSLVKGIAAHVAS
    • Sequence Length

    • 23
    • Evidence code

    • Protein level
    • Biological Activity

    • Antibacterial, Anti-Gram-, Antimicrobial
    • Target Organism

      • Gram-negative bacterium: Escherichia coli (MIC=25 µM).
    • Hemolytic activity

    • Unknown
    • Binding Target

    • Unknown
    • Structure

    • Alpha helix
    • Structure Description

    • Solution structural studies in the membrane mimicking environments, sodium dodecyl sulphate (SDS), dodecylphosphocholine (DPC), and 1,2-dihexanoyl-sn-glycero-3-phosphatidylcholine (DHPC) micelles, indicate that these peptides display an alpha helical structure between residues Lys(3) and Val(21).(Ref.1)
    • PDB ID

    • 2L5R resolved by NMR.
    • DRAMP01085 helical wheel diagram
    • 2L5R-> 
    • Formula

    • C104H176N28O28
    • Absent Amino Acids

    • CDMNPQRTWY
    • Common Amino Acids

    • AG
    • Mass

    • 2659.33
    • PI

    • 10.51
    • Basic Residues

    • 4
    • Acidic Residues

    • 1
    • Hydrophobic Residues

    • 12
    • Boman Index

    • 11.74
    • Hydrophobicity

    • 74.78
    • Aliphatic Index

    • 127.39
    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 0
    • Absorbance 280nm

    • 0
    • Polar Residues

    • 6

DRAMP01085

DRAMP01085 chydropathy plot
    • Function

    • Alyteserin-1c shows selective growth-inhibitory activity against Gram-negative bacteria.
  • Literature 1
    • Reference

    • Biochim Biophys Acta. 2011 Aug;1808(8):1975-1984.
    • Author

    • Subasinghage AP, O'Flynn D, Conlon JM, Hewage CM.
    • Title

    • Conformational and membrane interaction studies of the antimicrobial peptide alyteserin-1c and its analogue [E4K]alyteserin-1c.
  • Literature 2
    • Reference

    • Peptides. 2009 Jun;30(6):1069-1073.
    • Author

    • Conlon JM, Demandt A, Nielsen PF, Leprince J, Vaudry H, Woodhams DC.
    • Title

    • The alyteserins: two families of antimicrobial peptides from the skin secretions of the midwife toad Alytes obstetricans (Alytidae).