General Information

    • DRAMP ID

    • DRAMP01096

    • Peptide Name

    • Bombinin-like peptide 2 (BLP-2; toads, amphibians, animals)

    • Source

    • Bombina orientalis (Oriental fire-bellied toad)

    • Family

    • Belongs to the bombinin family

    • Gene

    • Not found

    • Sequence

    • GIGSAILSAGKSALKGLAKGLAEHFAN

    • Sequence Length

    • 27

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.1744108]Gram-negative bacterium: 58% inhibition Neisseria meningitidis 118V C (MIC=20 µg/ml), 98% inhibition Neisseria meningitidis 15240 IAI (MIC=20 µg/ml), 98% inhibition Neisseria meningitidis 126E IC1 (MIC=20 µg/ml), 97% inhibition Neisseria gonorrhoeoe F62 (MIC=20 µg/ml), 99% inhibition Neisseria Iactamica 15323 (MIC=20 µg/ml), 100% inhibition Neisseria Iactamica 15215A (MIC=20 µg/ml), 95% inhibition Neisseria cinerea 15461 (MIC=20 µg/ml).

    • Hemolytic Activity

      • [Ref:1744108] No significant hemolytic activity was found which suggests a selectivity for prokaryotic over eukaryotic membranes.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01096 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP01096.

Physicochemical Information

    • Formula

    • C115H193N33O34

    • Absent Amino Acids

    • CDMPQRTVWY

    • Common Amino Acids

    • A

    • Mass

    • 2582

    • PI

    • 9.7

    • Basic Residues

    • 4

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +3

    • Boman Index

    • 3.1

    • Hydrophobicity

    • 0.426

    • Aliphatic Index

    • 108.89

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 9

DRAMP01096

DRAMP01096 chydropathy plot

Comments Information

    • Function

    • Has antimicrobial activity, but no hemolytic activity. Preference on killing Gram-negative non-enteric bacteria.

    • Tissue specificity

    • Expressed by the skin glands.

Literature Information

  • ·Literature 1

    • Title

    • Bombinin-like peptides with antimicrobial activity from skin secretions of the Asian toad, Bombina orientalis.

    • Reference

    • J. Biol. Chem. 1991; 266: 23103-23111.

    • Author

    • Gibson BW, Tang D, Mandrell R, Kelly M, Spindel ER.