• DRAMP ID

    • DRAMP01098
    • Peptide Name

    • Bombinin-like peptide 3 (BLP-3; toads, amphibians, animals)
    • Source

    • Bombina orientalis (Oriental fire-bellied toad)
    • Family

    • Belongs to the bombinin family
    • Gene

    • Unknown
    • Sequence

    • GIGAAILSAGKSALKGLAKGLAEHF
    • Sequence Length

    • 25
    • Evidence code

    • Protein level
    • Biological Activity

    • Antibacterial, Anti-Gram-, Antimicrobial
    • Target Organism

      • [Ref.1744108]Gram-postive bacterium:Bacillus megaterium BmII (MIC=0.3 µM), Staphylococcus aureus Cowan1 (MIC=1.7 µM);
      • Gram-negative bacterium:59% inhibition Neisseria meningitidis 118V C (MIC=20 µg/ml), 99% inhibition Neisseria meningitidis 15240 IAI (MIC=20 µg/ml), 99% inhibition Neisseria meningitidis 126E IC1 (MIC=20 µg/ml), 99% inhibition Neisseria gonorrhoeoe F62 (MIC=20 µg/ml), 97% inhibitionNeisseria Iactamica 15323 (MIC=20 µg/ml), 100% inhibition Neisseria Iactamica 15215A (MIC=20 µg/ml), 97% inhibition Neisseria cinerea 15461 (MIC=20 µg/ml), Escherichia coli D21 (MIC=1.7 µM), Escherichia coli D22 (MIC=1.5 µM), Yersinia pseudotubercolosis (MIC=0.6 µM), Pseudomonas aeruginosa ATCC 15692 (MIC=9.2 µM);
      • Fungus:Candida albicans (MIC=0.4 µM).
    • Hemolytic activity

      • [Ref:1744108]Non-hemolytic activity
    • Binding Target

    • Unknown
    • Structure

    • Alpha helix
    • Structure Description

    • Unknown
    • DRAMP01098 helical wheel diagram
    • Formula

    • C108H182N30O30
    • Absent Amino Acids

    • CDMNPQRTVWY
    • Common Amino Acids

    • A
    • Mass

    • 2809.12
    • PI

    • 10.51
    • Basic Residues

    • 4
    • Acidic Residues

    • 1
    • Hydrophobic Residues

    • 13
    • Boman Index

    • 13.14
    • Hydrophobicity

    • 63.2
    • Aliphatic Index

    • 117.6
    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 0
    • Absorbance 280nm

    • 0
    • Polar Residues

    • 7

DRAMP01098

DRAMP01098 chydropathy plot
    • Function

    • Has antimicrobial activity, but no hemolytic activity. Preference on killing Gram-negative non-enteric bacteria.
    • Tissue specificity

    • Expressed by the skin glands.
  • Literature 1
    • Reference

    • J Biol Chem. 1991 Dec 5;266(34):23103-23111.
    • Author

    • Gibson BW, Tang DZ, Mandrell R, Kelly M, Spindel ER.
    • Title

    • Bombinin-like peptides with antimicrobial activity from skin secretions of the Asian toad, Bombina orientalis.