• DRAMP ID

    • DRAMP01152
    • Peptide Name

    • Uperin-3.6 (toads, amphibians, animals)
    • Source

    • Uperoleia mjobergii (Australian toadlet)
    • Family

    • Unknown
    • Gene

    • Unknown
    • Sequence

    • GVIDAAKKVVNVLKNLP
    • Sequence Length

    • 17
    • Evidence code

    • Protein level
    • Biological Activity

    • Antibacterial, Anti-Gram+, Antimicrobial
    • Target Organism

      • Gram-positive bacteria: Bacillus cereus (MIC=25 µg/ml), Lactococcus lactis (MIC=3 µg/ml), Listeria innocua (MIC=50 µg/ml), Micrococcus luteus (MIC=25 µg/ml), Staphylococcus aureus (MIC=25 µg/ml), Staphylococcus epidermidis (MIC=12.5 µg/ml), Streptococcus uberis (MIC=12.5 µg/ml).(Ref.2)
    • Hemolytic activity

    • Unknown
    • Binding Target

    • Unknown
    • Structure

    • Alpha helix
    • Structure Description

    • In 50% (by vol.) trifluoroethanol, an NMR study and structure calculations indicate that uperin 3.6 adopts a well-defined amphipathic alpha-helix with distinct hydrophilic and hydrophobic faces.(Ref.1)
    • DRAMP01152 helical wheel diagram
    • Formula

    • C81H144N22O22
    • Absent Amino Acids

    • CEFHMQRSTWY
    • Common Amino Acids

    • V
    • Mass

    • 2063.76
    • PI

    • 10.51
    • Basic Residues

    • 3
    • Acidic Residues

    • 1
    • Hydrophobic Residues

    • 9
    • Boman Index

    • -3.17
    • Hydrophobicity

    • 48.82
    • Aliphatic Index

    • 148.82
    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 0
    • Absorbance 280nm

    • 0
    • Polar Residues

    • 3

DRAMP01152

    • Function

    • Shows antibacterial activity gaginst several Gram-positive bacteria. This peptide could inhibit HIV infection at a concentration that is also toxic to T cells (J Virol 2005; 79
  • Literature 1
    • Reference

    • J Pept Res. 1999 Aug;54(2):137-145.
    • Author

    • Chia BC, Carver JA, Mulhern TD, Bowie JH.
    • Title

    • The solution structure of uperin 3.6, an antibiotic peptide from the granular dorsal glands of the Australian toadlet, Uperoleia mjobergii.
  • Literature 2
    • Reference

    • Peptides. 2004 Jun;25(6):1035-1054.
    • Author

    • Apponyi MA, Pukala TL, Brinkworth CS, Maselli VM, Bowie JH, Tyler MJ, Booker GW, Wallace JC, Carver JA, Separovic F, Doyle J, Llewellyn LE.
    • Title

    • Host-defence peptides of Australian anurans: structure, mechanism of action and evolutionary significance.