General Information
-
DRAMP ID
- DRAMP01162
-
Peptide Name
- Buforin-1 (Buforin I; Fragment of Histone H2A; toads, amphibians, animals)
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Source
- Bufo gargarizans (Asian toad) (Bufo bufo gargarizans)
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Family
- Belongs to the histone H2A family
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Gene
- Not found
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Sequence
- AGRGKQGGKVRAKAKTRSSRAGLQFPVGRVHRLLRKGNY
-
Sequence Length
- 39
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Swiss_Prot Entry
- P55897
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Evidence code
- Protein level
Activity Information
-
Biological Activity
- Antibacterial, Antifungal, Anti-Gram+, Anti-Gram-, Antimicrobial
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Target Organism
-
- Gram-positive bacteria: Bacillus subtilis (MIC=4 µg/ml), Staphylococcus aureus (MIC=4 µg/ml), Streptococcus mutans (MIC=8 µg/ml), Streptococcus pneumoniae (MIC=4 µg/ml), Pseudomonas putida (MIC=4 µg/ml);
- Gram-negative bacteria: Escherichia coli (MIC=8 µg/ml), Salmonella typhimurium (MIC=4 µg/ml), Serratia sp. (MIC=8 µg/ml).
- Fungi: Candida albicans (MIC=4 µg/ml), Cryptococcus neoformans (MIC=4 µg/ml), Saccharomyces cerevisiae (MIC=4 µg/ml).
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Hemolytic Activity
-
- Not found
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Cytotoxicity
-
- Not included yet
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Binding Target
- DNA
Structure Information
-
Linear/Cyclic
- Not included yet
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N-terminal Modification
- Not included yet
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C-terminal Modification
- Not included yet
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Other Modifications and Unusual Amino Acids
- Not included yet
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Stereochemistry
- Not included yet
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Structure
- Not found
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Structure Description
- Not found
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PDB ID
- None
-
-
Physicochemical Information
-
Formula
- C184H318N70O47
Absent Amino Acids
- CDEIMW
Common Amino Acids
- GR
Mass
- 4940.96
PI
- 12.91
Basic Residues
- 13
Acidic Residues
- 0
Hydrophobic Residues
- 11
-
Boman Index
- -120.4
Hydrophobicity
- -0.992
Aliphatic Index
- 62.56
Half Life
-
- Mammalian:4.4 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 1490
Absorbance 280nm
- 39.21
Polar Residues
- 12
DRAMP01162

Comments Information
Function
- Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
PTM
- Monoubiquitination of C-terminus gives a specific tag for epigenetic transcriptional repression. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties.
Literature Information
- ·Literature 1
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Title
- A novel antimicrobial peptide from Bufo bufo gargarizans.
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Pubmed ID
- 8573171
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Reference
- Biochem Biophys Res Commun. 1996 Jan 5;218(1):408-413.
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Author
- Park C.B, Kim M.S, Kim S.C.
- ·Literature 2
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Title
- Solution structure of an antimicrobial peptide buforin II.
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Pubmed ID
- 8946958
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Reference
- FEBS Lett. 1996 Nov 25;398(1):87-90.
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Author
- Yi G.-S, Park C.B, Kim S.C, Cheong C.