• DRAMP ID

    • DRAMP01163
    • Peptide Name

    • Buforin-2 (Buforin II; Fragment of Histone H2A; toads, amphibians, animals)
    • Source

    • Bufo gargarizans (Asian toad) (Bufo bufo gargarizans)
    • Family

    • Belongs to the histone H2A family
    • Gene

    • Unknown
    • Sequence

    • TRSSRAGLQFPVGRVHRLLRK
    • Sequence Length

    • 21
    • Evidence code

    • Protein level
    • Biological Activity

    • Antibacterial, Antifungal, Anti-Gram+, Anti-Gram-, Antimicrobial
    • Target Organism

      • Gram-positive bacteria: Bacillus subtilis (MIC=2 µg/ml), Staphylococcus aureus (MIC=4 µg/ml), Streptococcus mutans (MIC=2 µg/ml), Streptococcus pneumoniae (MIC=4 µg/ml), Pseudomonas putida (MIC=2 µg/ml);
      • Gram-negative bacteria: Escherichia coli (MIC=4 µg/ml), Salmonella typhimurium (MIC=1 µg/ml), Serratia sp. (MIC=4 µg/ml).
      • Fungi: Candida albicans (MIC=1 µg/ml), Cryptococcus neoformans (MIC=1 µg/ml), Saccharomyces cerevisiae (MIC=1 µg/ml).
    • Hemolytic activity

    • Unknown
    • Binding Target

    • DNA
    • Structure

    • Alpha helix
    • Structure Description

    • Unknown
    • DRAMP01163 helical wheel diagram
    • Formula

    • C106H184N40O26
    • Absent Amino Acids

    • CDEIMNWY
    • Common Amino Acids

    • R
    • Mass

    • 2791.78
    • PI

    • 13.1
    • Basic Residues

    • 7
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 7
    • Boman Index

    • -70.21
    • Hydrophobicity

    • -63.81
    • Aliphatic Index

    • 88.1
    • Half Life

      • Mammalian:7.2 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 0
    • Absorbance 280nm

    • 0
    • Polar Residues

    • 5

DRAMP01163

DRAMP01163 chydropathy plot
    • Function

    • Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Both buforin II and its derivative buforin IIb (sequence
    • PTM

    • Monoubiquitination of C-terminus gives a specific tag for epigenetic transcriptional repression. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties.
  • Literature 1
    • Reference

    • Biochem Biophys Res Commun. 1996 Jan 5;218(1):408-413.
    • Author

    • Park C.B, Kim M.S, Kim S.C.
    • Title

    • A novel antimicrobial peptide from Bufo bufo gargarizans.
  • Literature 2
    • Reference

    • FEBS Lett. 1996 Nov 25;398(1):87-90.
    • Author

    • Yi G.-S, Park C.B, Kim S.C, Cheong C.
    • Title

    • Solution structure of an antimicrobial peptide buforin II.