General Information

    • DRAMP ID

    • DRAMP01167

    • Peptide Name

    • Hylaseptin-P1 (HSP1)

    • Source

    • Hyla punctata (Polka-dot tree frog) (Spotted tree frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GILDAIKAIAKAAG

    • Sequence Length

    • 14

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.14715660]Gram-positive bacterium: Staphylococcus aureus ATCC 25923 (MIC=6.1-8.0 µg/ml);
      • Gram-negative bacteria: Pseudomonas aeruginosa ATCC 27853 (MIC=48.8-64.0 µg/ml), Escherichia coli ATCC 25922 (MIC=24.4-32.0 µg/ml).

    • Hemolytic Activity

      • [Ref.14715660] HSP1 MIC concentration (256 μg/mL = 195.2 μM) produced 4.7% hemolysis against human erythrocytes.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • [Ref.11297740] Free / [Ref.32828849] Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01167 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP01167.

Physicochemical Information

    • Formula

    • C59H106N16O17

    • Absent Amino Acids

    • CEFHMNPQRSTVWY

    • Common Amino Acids

    • A

    • Mass

    • 1311.59

    • PI

    • 8.59

    • Basic Residues

    • 2

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +1

    • Boman Index

    • 10.79

    • Hydrophobicity

    • 1.014

    • Aliphatic Index

    • 147.14

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 2

DRAMP01167

Comments Information

    • Function

    • Has antibacterial and antifungal activity. A weak hemolytic activity has been detected.

Literature Information

  • ·Literature 1

    • Title

    • The NMR-derived solution structure of a new cationic antimicrobial peptide from the skin secretion of the anuran Hyla punctata.

    • Reference

    • J Biol Chem. 2004 Mar 26;279(13):13018-13026.

    • Author

    • Prates MV, Sfor§a ML, Regis WC, Leite JR, Silva LP, Pertinhez TA, Araºjo AL, Azevedo RB, Spisni A, Bloch C Jr.
  • ·Literature 2

    • Title

    • Membrane interactions of the anuran antimicrobial peptide HSP1-NH 2: Different aspects of the association to anionic and zwitterionic biomimetic systems

    • Reference

    • Biochim Biophys Acta Biomembr . 2021 Jan 1;1863(1):183449. doi: 10.1016/j.bbamem.2020.183449. Epub 2020 Aug 21.

    • Author

    • Isabela P Gomes 1, Talita L Santos 1, Amanda N de Souza 1, Lúcio O Nunes, Gabriele A Cardoso, Carolina O Matos, Lívia M F Costa, Luciano M Lião, Jarbas M Resende, Rodrigo M Verly