General Information

    • DRAMP ID

    • DRAMP01170

    • Peptide Name

    • Distinctin 2 (Frogs, amphibians, animals)

    • Source

    • Phyllomedusa distincta (Tree frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • NLVSGLIEARKYLEQLHRKLKNCKV

    • Sequence Length

    • 25

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Note: Distinctin chain 2 links to Distinctin chain 1 to form a heterodimeric peptide to exert antibacterial function.
      • Gram-positive bacteria: Enterococcus faecalis (MIC=14.5 µM), Staphylococcus aureus (MIC=28.0 µM);
      • Gram-negative bacteria: Pseudomonas aeruginosa (MIC=28.0 µM), Escherichia coli (MIC=14.5 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • There is an intermolecular disulphide bridge between Distinctin chain 1 (Cys19) and Distinctin chain 2 (Cys22).

    • Stereochemistry

    • L

    • Structure

    • Alpha helix (1 helices; 17 residues)

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01170 helical wheel diagram

    • PDB ID

    • 1XKM resolved by NMR
  • 1XKM-> 

    • Predicted Structure

Physicochemical Information

    • Formula

    • C131H226N40O35S

    • Absent Amino Acids

    • DFMPTW

    • Common Amino Acids

    • L

    • Mass

    • 2953.54

    • PI

    • 9.86

    • Basic Residues

    • 7

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +5

    • Boman Index

    • -51.05

    • Hydrophobicity

    • -0.464

    • Aliphatic Index

    • 120.8

    • Half Life

      • Mammalian:1.4 hour
      • Yeast:3 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 1490

    • Absorbance 280nm

    • 62.08

    • Polar Residues

    • 6

DRAMP01170

DRAMP01170 chydropathy plot

Comments Information

    • Comment

    • Distinctin is constituted of two different polypeptide chains connected by an intermolecular disulphide bridge. The entry is the chain 2 of Distinctin

Literature Information

  • ·Literature 1

    • Title

    • A novel heterodimeric antimicrobial peptide from the tree-frog Phyllomedusa distincta.

    • Reference

    • FEBS Lett. 2001 Apr 6;494(1-2):85-89.

    • Author

    • Batista CV, Scaloni A, Rigden DJ, Silva LR, Rodrigues Romero A, Dukor R, Sebben A, Talamo F, Bloch C.