• DRAMP ID

    • DRAMP01214
    • Peptide Name

    • Kassinatuerin-2Ma (Frogs, amphibians, animals)
    • Source

    • Kassina maculata (African hyperoliid frog)
    • Family

    • Unknown
    • Gene

    • Unknown
    • Sequence

    • FLGAIAAALPHVINAVTNAL
    • Sequence Length

    • 20
    • Swiss_Prot Entry

    • No entry found
    • Evidence code

    • Unknown
    • Biological Activity

    • Antibacterial, Anti-Gram+, Anti-Gram-, Antimicrobial
    • Target Organism

      • [Ref.19427345]Gram-positive bacterium: Staphylococcus aureus (NCTC 10788) (MIC=16 µM);
      • Gram-negative bacterium: Escherichia coli (NCTC 10418) (MIC>200 µM) (MIC=120 µM);
      • Yeast: Candida albicans (NCPF 1467) (MIC=120 µM).
    • Hemolytic activity

      • [Ref:19427345]18% hemolytic activity at 16 μM, 45–50% hemolytic activity at 120 μM against horse erythrocytes
    • Binding Target

    • Unknown
    • Structure

    • Alpha helix
    • Structure Description

    • Secondary structure prediction analysis revealed that virtually the entire structure of each kassinatuerin-2 related peptide is composed of alpha-helix, the helix was not amphipathic like many other amphibian skin antimicrobial peptides, in which there is a hydrophobic face and a positively charged faces the latter presumably interacting with the negatively charged glycocalyx of cell membranes.
    • DRAMP01214 helical wheel diagram
    • Formula

    • C92H150N24O24
    • Absent Amino Acids

    • CDEKMQRSWY
    • Common Amino Acids

    • A
    • Mass

    • 2315.47
    • PI

    • 7.55
    • Basic Residues

    • 1
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 14
    • Boman Index

    • 26.95
    • Hydrophobicity

    • 147.5
    • Aliphatic Index

    • 156.5
    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min
    • Extinction Coefficient Cystines

    • 0
    • Absorbance 280nm

    • 0
    • Polar Residues

    • 4

DRAMP01214

DRAMP01214 chydropathy plot
    • Function

    • Natural kassinatuerin-2Ma and their synthetic replicates possessed relatively potent growth inhibitory activity against the Gram-positive bacterium, S. aureus, but were ineffective against the Gram-negative bacterium, E. coli at concentrations up to 200 µM and were relatively ineffective against the pathogenic yeast, C. albicans (120 µM). Also possessed hemolytic activity as demonstrated using horse erythrocytes.
  • Literature 1
    • Reference

    • Peptides. 2009 Aug;30(8):1428-1433.
    • Author

    • Wang L, Zhou M, McGrath S, Chen T, Gorman SP, Walker B, Shaw C.
    • Title

    • A family of kassinatuerin-2 related peptides from the skin secretion of the African hyperoliid frog, Kassina maculata.