General Information

    • DRAMP ID

    • DRAMP01217

    • Peptide Name

    • Kassinatuerin-2Md (Frogs, amphibians, animals)

    • Source

    • Kassina maculata (African hyperoliid frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • IIGAIAAALPHVINAIKNTF

    • Sequence Length

    • 20

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

      • [Ref.19427345]Gram-positive bacterium: Staphylococcus aureus

    • Hemolytic Activity

      • [Ref:19427345]18% hemolytic activity at 16 μM, 45–50% hemolytic activity at 120 μM against horse erythrocytes

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix

    • Structure Description

    • Secondary structure prediction analysis revealed that virtually the entire structure of each kassinatuerin-2 related peptide is composed of alpha-helix, the helix was not amphipathic like many other amphibian skin antimicrobial peptides, in which there is a hydrophobic face and a positively charged faces the latter presumably interacting with the negatively charged glycocalyx of cell membranes.

    • Helical Wheel Diagram

    • DRAMP01217 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP01217.

Physicochemical Information

    • Formula

    • C96H159N25O24

    • Absent Amino Acids

    • CDEMQRSWY

    • Common Amino Acids

    • AI

    • Mass

    • 2047.47

    • PI

    • 8.76

    • Basic Residues

    • 2

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +2

    • Boman Index

    • 20.47

    • Hydrophobicity

    • 1.275

    • Aliphatic Index

    • 156.5

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 4

DRAMP01217

DRAMP01217 chydropathy plot

Comments Information

    • Function

    • Has relatively potent growth inhibitory activity against the Gram-positive bacterium, S. aureus, but were ineffective against the Gram-negative bacterium, E. coli and were relatively ineffective against the pathogenic yeast, C. albicans. Has hemolytic activity as demonstrated using horse erythrocytes.

Literature Information

  • ·Literature 1

    • Title

    • A family of kassinatuerin-2 related peptides from the skin secretion of the African hyperoliid frog, Kassina maculata.

    • Reference

    • Peptides. 2009 Aug;30(8):1428-1433.

    • Author

    • Wang L, Zhou M, McGrath S, Chen T, Gorman SP, Walker B, Shaw C.