• DRAMP ID

    • DRAMP01222
    • Peptide Name

    • Palustrin-2AJ1 (PL2AJ1; Frogs, amphibians, animals)
    • Source

    • Amolops jingdongensis (Chinese torrent frog)
    • Family

    • N/A
    • Gene

    • N/A
    • Sequence

    • GFMDTAKNVAKNVAVTLIDKLRCKVTGGC
    • Sequence Length

    • 29
    • Evidence code

    • Protein level
    • Biological Activity

    • Antibacterial, Anti-Gram+, Anti-Gram-, Antimicrobial
    • Target Organism

      • [Ref.21816202]Gram-positive bacteria: Staphylococcus aureus ATCC25923 (MIC=20 µM), Enterococcus faecalis ATCC29212 (MIC=20 µM);
      • Gram-negative bacteria: Escherichia coli ATCC25922 (MIC=13 µM), Bacillus pyocyaneus CMCCB1010 (MIC=20 µM), Pseudomonas aeruginosa ATCC27853 (MIC=33 µM), Bacillus dysenteriae (MIC=50 µM).
    • Hemolytic Activity

      • [Ref:21816202]IC50=135 μM against human and rabbit erythrocytes
    • Cytotoxicity

    • Binding Target

    • N/A
    • Linear/Cyclic/Branched

    • N-terminal modification

    • C-terminal modification

    • Other modifications

    • Stereochemistry

    • Structure

    • N/A
    • Structure Description

    • N/A
    • DRAMP01222 helical wheel diagram
    • Formula

    • C131H226N38O39S3
    • Absent Amino Acids

    • EHPQSWY
    • Common Amino Acids

    • KV
    • Mass

    • 3553.29
    • PI

    • 9.6
    • Basic Residues

    • 5
    • Acidic Residues

    • 2
    • Hydrophobic Residues

    • 11
    • Boman Index

    • -28.49
    • Hydrophobicity

    • 22.76
    • Aliphatic Index

    • 90.69
    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 125
    • Absorbance 280nm

    • 4.46
    • Polar Residues

    • 10

DRAMP01222

DRAMP01222 chydropathy plot
    • Function

    • Displays broad-spectrum antibacterial activity against a range of Gram-positive and Gram-negative bacteria. Has hemolytic activity, low cytotoxicity and low antioxidant activity.
    • Tissue specificity

    • Expressed by the skin glands.
    • PTM

    • One disulfide bond (C23-C29) and Cleavage on pair of basic residues.
  • Literature 1
    • Reference

    • Biochimie. 2012 Feb;94(2):328-334.
    • Author

    • Chen Z, Yang X, Liu Z, Zeng L, Lee W, Zhang Y.
    • Title

    • Two novel families of antimicrobial peptides from skin secretions of the Chinese torrent frog, Amolops jingdongensis.