General Information

    • DRAMP ID

    • DRAMP01224

    • Peptide Name

    • Palustrin-2AR (Palustrin-2ARa; Ranatuerin-2SEa; Frogs, amphibians, animals)

    • Source

    • Rana areolata (North American crawfish frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GFISTVKNLATNVAGTVIDTIKCKVTGGC

    • Sequence Length

    • 29

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-

    • Target Organism

      • [Ref.12429503] Gram-negative bacterium: Escherichia coli (MIC=110 μM)

    • Hemolytic Activity

      • [Ref.12429503] HC50>300 μM against human erythrocytes

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys23 and Cys29)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys23 and Cys29.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01224 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C126H216N34O40S2

    • Absent Amino Acids

    • EHMPQRWY

    • Common Amino Acids

    • T

    • Mass

    • 2911.42

    • PI

    • 8.86

    • Basic Residues

    • 3

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +2

    • Boman Index

    • -6.14

    • Hydrophobicity

    • 0.6

    • Aliphatic Index

    • 100.69

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 4.46

    • Polar Residues

    • 14

DRAMP01224

DRAMP01224 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-negative bacterium Escherichia coli. Has hemolytic activity.

Literature Information

  • ·Literature 1

    • Title

    • Antimicrobial peptides and protease inhibitors in the skin secretions of the crawfish frog, Rana areolata.

    • Reference

    • Biochim Biophys Acta. 2002 Nov 19;1601(1):55-63.

    • Author

    • Ali MF, Lips KR, Knoop FC, Fritzsch B, Miller C, Conlon JM.