• DRAMP ID

    • DRAMP01347
    • Peptide Name

    • Prepromelittin-related peptide (Frogs, amphibians, animals)
    • Source

    • Rana tagoi (Tago frog)
    • Family

    • Not found
    • Gene

    • prMRP
    • Sequence

    • AIGSILGALAKGLPTLISWIKNR
    • Sequence Length

    • 23
    • Protein Existence

    • Transcript level
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
    • Target Organism

      • [Ref.12804591]Gram-positive bacterium: Staphylococcus aureus (MIC=3.5 µM);
      • Gram-negative bacterium: Escherichia coli (MIC=20 µM).
      • Yeast: Candida albicans (MIC=19 µM).
      • EL4 T-lymphoma cells (MIC=14 µM).
      • [Ref.27271216]Gram-positive bacteria : Staphylococcuss aureus (MIC=6.25 μM), Staphylococcus epidermidis (MIC=3.13 μM), Bacillus subtilis (MIC=3.13 μM);
      • Gram-negative bacteria : Escherichia coli (MIC=25 μM), Pseudomonas aeruginosa (MIC=12.5 μM), Klebsiella pneumoniae (MIC=12.5 μM)
    • Hemolytic Activity

      • [Ref.27271216] 100% hemolysis at 20 μM against human red blood cells
    • Cytotoxicity

      • Not included yet
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Not included yet
    • N-terminal Modification

    • Not included yet
    • C-terminal Modification

    • Not included yet
    • Other Modifications and Unusual Amino Acids

    • Not included yet
    • Stereochemistry

    • Not included yet
    • Structure

    • Alpha helix
    • Structure Description

    • Not found
    • PDB ID

    • 6dst resolved by NMR (related PDB_ID)
    • DRAMP01347 helical wheel diagram
    • Formula

    • C111H190N30O28
    • Absent Amino Acids

    • CDEFHMQVY
    • Common Amino Acids

    • IL
    • Mass

    • 2392.91
    • PI

    • 11.17
    • Basic Residues

    • 3
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 12
    • Net Charge

    • +3
    • Boman Index

    • 7.91
    • Hydrophobicity

    • 0.73
    • Aliphatic Index

    • 148.7
    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 5500
    • Absorbance 280nm

    • 250
    • Polar Residues

    • 7

DRAMP01347

DRAMP01347 chydropathy plot
    • Function

    • Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against C. albicans.
  • ·Literature 1
    • Title

    • A melittin-related peptide from the skin of the Japanese frog, Rana tagoi, with antimicrobial and cytolytic properties
    • Reference

    • Biochem Biophys Res Commun. 2003 Jun 27;306(2):496-500.
    • Author

    • Conlon JM, Sonnevend A, Patel M, Camasamudram V, Nowotny N, Zilahi E, Iwamuro S, Nielsen PF, Pál T.2003.
  • ·Literature 2
    • Title

    • Design of an α-helical antimicrobial peptide with improved cell selective and potent anti-biofilm activity
    • Reference

    • Sci Rep. 2016 Jun 8;6:27394. doi: 10.1038/srep27394.
    • Author

    • Shi-KunZhang, Jin-wen Song, FengGong, Su-Bo Li, Hong-Yu Chang, Hui-MinXie, Hong-WeiGao, Ying-XiaTan & Shou-Ping Ji