• DRAMP ID

    • DRAMP01353
    • Peptide Name

    • Tigerinin-4 (Frogs, amphibians, animals)
    • Source

    • Rana tigerina (Indian bull frog)
    • Family

    • Not found
    • Gene

    • Not found
    • Sequence

    • RVCYAIPLPIC
    • Sequence Length

    • 11
    • Protein Existence

    • Protein level
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
    • Target Organism

      • Gram-positive bacterium: Staphylococcus aureus ATCC 8530 (MIC=50 µg/ml);
      • Gram-negative bacteria: Escherichia coli (MIC=60 µg/ml), Pseudomonas putida NCIM 2102 (MIC=60 µg/ml).
      • Fungi: Saccharomyces cerevisiae ATCC 8530 (MIC=100 µg/ml).
    • Hemolytic Activity

      • [Ref.11031261] It exhibits no hemolytic activity up to a concentration of 200 μg/ml against rat erythrocytes.
    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented
    • Binding Target

    • Cell membrane
    • Linear/Cyclic

    • Cyclic
    • N-terminal Modification

    • Free
    • C-terminal Modification

    • Amidation;Cyclization (Cys3 and Cys11)
    • Other Modifications and Unusual Amino Acids

    • Disulfide bond between Cys3 and Cys11.
    • Stereochemistry

    • L
    • Structure

    • Beta strand-turn
    • Structure Description

    • Not found
    • PDB ID

    • None
    • DRAMP01353 helical wheel diagram
    • Formula

    • C57H94N14O13S2
    • Absent Amino Acids

    • DEFGHKMNQSTW
    • Common Amino Acids

    • CIP
    • Mass

    • 1247.58
    • PI

    • 8.06
    • Basic Residues

    • 1
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 5
    • Net Charge

    • +1
    • Boman Index

    • 8.11
    • Hydrophobicity

    • 1.346
    • Aliphatic Index

    • 141.82
    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min
    • Extinction Coefficient Cystines

    • 1615
    • Absorbance 280nm

    • 161.5
    • Polar Residues

    • 3

DRAMP01353

    • Function

    • Has antibacterial against Gram-positive and Gram-negative bacteria. Also has antifungal activity against C. albicans.
  • ·Literature 1
    • Title

    • Tigerinins: novel antimicrobial peptides from the Indian frog Rana tigerina.
    • Reference

    • J Biol Chem. 2001 Jan 26;276(4):2701-2707.
    • Author

    • Sai KP, Jagannadham MV, Vairamani M, Raju NP, Devi AS, Nagaraj R, Sitaram N.