• DRAMP ID

    • DRAMP01354
    • Peptide Name

    • Peptide leucine arginine (pLR; Frogs, amphibians, animals)
    • Source

    • Rana pipiens (Northern leopard frog)
    • Family

    • Unknown
    • Gene

    • Unknown
    • Sequence

    • LVRGCWTKSYPPKPCFVR
    • Sequence Length

    • 18
    • Evidence code

    • Protein level
    • Biological Activity

    • Antibacterial, Antifungal, Anti-Gram+, Antimicrobial
    • Target Organism

      • [Ref.11099505]Gram-positive bacteria: Bacillus megaterium Bm11 (MIC=20 µM), Staphylococcus lentus (MIC=50 µM), Micrococcus luteus (MIC=10 µM);
      • Fungi: Candida tropicalis (MIC=11 µM), Candida guillermondii (MIC=6.6 µM), Phytophthora nicotianae spores (MIC=75 µM).
    • Hemolytic activity

      • [Ref:11099505]2.5% hemolytic activity at 100 μM against human red blood
    • Binding Target

    • Unknown
    • Structure

    • Unknown
    • Structure Description

    • Unknown
    • DRAMP01354 helical wheel diagram
    • Formula

    • C99H153N27O22S2
    • Absent Amino Acids

    • ADEHIMNQ
    • Common Amino Acids

    • P
    • Mass

    • 2440.98
    • PI

    • 10.26
    • Basic Residues

    • 4
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 5
    • Boman Index

    • -25.24
    • Hydrophobicity

    • -31.67
    • Aliphatic Index

    • 53.89
    • Half Life

      • Mammalian:5.5 hour
      • Yeast:3 min
      • E.coli:2 min
    • Extinction Coefficient Cystines

    • 7115
    • Absorbance 280nm

    • 418.53
    • Polar Residues

    • 6

DRAMP01354

DRAMP01354 chydropathy plot
    • Function

    • Mast cell degranulating peptide. Antiproliferative activity against human breast and ovarian tumor cell lines in vitro. Inhibits granulopoiesis in rat in vitro. Causes histamine release from rat peritoneal mast cells in vitro. Has antibacterial activity against Gram-positive bacteria
  • Literature 1
    • Reference

    • J. Biol. Chem. 2001;276:10145-10152.
    • Author

    • Salmon A.L, Cross L.J.M, Irvine A.E, Lappin T.R.J, Dathe M, Krause G, Canning P, Thim L, Beyermann M, Rothemund S, Bienert M, Shaw C.
    • Title

    • Peptide leucine arginine, a potent immunomodulatory peptide, isolated and structurally characterized from the skin of the Northern Leopard frog, Rana pipiens.