General Information

    • DRAMP ID

    • DRAMP01376

    • Peptide Name

    • Odorranain-F1 (OdF1; Frogs, amphibians, animals)

    • Source

    • Odorrana grahami (Yunnanfu frog) (Rana grahami)

    • Family

    • Belongs to the frog skin active peptide (FSAP) family. Brevinin subfamily.

    • Gene

    • Not found

    • Sequence

    • GFMDTAKNAAKNVAVTLLDNLKCKITKAC

    • Sequence Length

    • 29

    • Protein Existence

    • Transcript level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.17272268] Gram-negative bacterium: Escherichia coli (MIC=4.20 μg/ml);
      • Gram-positive bacteria: Staphylococcus aureus (MIC=2.10 μg/ml), Bacillus subtilis (MIC=8.40 μg/ml).
      • Yeast: Candida albicans (MIC=1.05 μg/ml).

    • Hemolytic Activity

      • [Ref.17272268] RCH=15.4 ± 3.5% against rabbit red blood cells at 50 μg/ml. RCH, red cell hemolysis.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys23 and Cys29)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys23 and Cys29.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Odorranain-F1 adopts 55%

    • Helical Wheel Diagram

    • DRAMP01376 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C133H229N37O40S3

    • Absent Amino Acids

    • EHPQRSWY

    • Common Amino Acids

    • AK

    • Mass

    • 3082.69

    • PI

    • 9.24

    • Basic Residues

    • 5

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 12

    • Net Charge

    • +3

    • Boman Index

    • -27.18

    • Hydrophobicity

    • 0.121

    • Aliphatic Index

    • 91.03

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 4.46

    • Polar Residues

    • 9

DRAMP01376

DRAMP01376 chydropathy plot

Comments Information

    • Function

    • Odorranain-F1 exhibites antimicrobial activities against the tested microbes including Gram-positive and Gram-negative bacteria and fungi. Also has hemolytic activity against rabbiit red cell.

Literature Information

  • ·Literature 1

    • Title

    • Anti-infection peptidomics of amphibian skin.

    • Reference

    • Mol Cell Proteomics. 2007 May;6(5):882-894.

    • Author

    • Li J, Xu X, Xu C, Zhou W, Zhang K, Yu H, Zhang Y, Zheng Y, Rees HH, Lai R, Yang D, Wu J.