• • DRAMP ID

  • DRAMP01445

• • Peptide Name

  • Nigrocin-2 (Nigrocin-2LVa; Frogs, amphibians, animals)

• • Source

  • Rana nigromaculata (Black-spotted frog)

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • GLLSKVLGVGKKVLCGVSGLC

• • Sequence Length

  • 21

• • UniProt Entry

  • P0C009

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

• • Target Organism

  • • [Ref.11682065]Gram-positive bacterium: Micrococcus luteus(MIC=2.5μg/ml);
    • Gram-negative bacteria: Shigella dysenteriae(MIC=10μg/ml), Klebsiella pneumoniae(MIC=10μg/ml), Pseudomonas aeruginosa(MIC=100μg/ml), Salmonella typhimurium(MIC=22.5μg/ml), Proteus mirabilis(MIC>200μg/ml), Serratia marcescens(MIC>200μg/ml);
    • Yeast: Candida albicans(MIC=150μg/ml).

• • Hemolytic Activity

  • • [Ref:11682065] It has 0.9% hemolytic activity at 100μg/ml against human red blood cells

• • Cytotoxicity

  • No cytotoxicity information found in the reference(s) presented

• • Binding Target

  • Cell membrane

• • Linear/Cyclic

  • Cyclic

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Cyclization (Cys15 and Cys21)

• • Nonterminal Modifications and Unusual Amino Acids

  • Disulfide bond between Cys15 and Cys21.

• • Stereochemistry

  • L

• • Structure

  • Alpha helix

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP01445.

DRAMP01445

• • Function

  • Thanks to its single linear amphipathic alpha-helix, may integrate into membrane phospholipids, leading to lysis of the membrane. Shows antibacterial activity against both Gram-positive and Gram-negative bacteria and against the fungus C. albicans. Has weak hemolytic activity.

• • Tissue specificity

  • Expressed by the skin dorsal glands.

• • PTM

  • Contains one disulfide bond 15-21. (By similarity)

• ·Literature 1

• • Title

  • Structural study of novel antimicrobial peptides, nigrocins, isolated from Rana nigromaculata.

• • Pubmed ID

  • 11682065

• • Reference

  • FEBS Lett. 2001 Oct 19;507(1):95-100.

• • Author

  • Park S, Park SH, Ahn HC, Kim S, Kim SS, Lee BJ, Lee BJ.