• • DRAMP ID

  • DRAMP01555

• • Peptide Name

  • Caerin-1.5 (Frogs, amphibians, animals)

• • Source

  • Litoria caerulea (Green tree frog)

• • Family

  • Belongs to the frog skin active peptide family (Caerin subfamily)

• • Gene

  • Not found

• • Sequence

  • GLLSVLGSVVKHVIPHVVPVIAEHL

• • Sequence Length

  • 25

• • UniProt Entry

  • P56230

• • Protein Existence

  • Protein level

• • SMILES

  • CC[C@@H](C)[C@H](NC(=O)[C@@H](NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)CN)C(C)C)C(C)C)C(C)C)C(C)C)[C@H](C)CC)C(C)C)C(C)C)C(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@@H](CC(C)C)C(N)=O

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-,Antiviral

• • Target Organism

  • • [Ref.15203252]Gram-positive bacteria:

      Target Organism	Activity
      Bacillus cereus	MIC=50 µg/ml
      Leuconostoc lactis	MIC=3 µg/ml
      Listeria innocua	MIC=50 µg/ml
      Micrococcus luteus	MIC=12 µg/ml
      Staphylococcus aureus	MIC=25 µg/ml
      Staphylococcus epidermis	MIC=25 µg/ml
      Streptococcus uberis (MIC=50 µg/ml);	-

    • Gram-negative bacterium:

      Target Organism	Activity
      Pasteurella multocida	MIC=25 µg/ml

    • [Ref.26026377]Virus:

      Target Organism	Activity
      HIV: inhibition of HIV Pseudovirus (PsV) infection in CD4+ T cells	IC50=3 µM

• • Hemolytic Activity

  • • No hemolysis information or data found in the reference(s) presented in this entry

• • Cytotoxicity

  • • [Ref.26026377]Human endocervical cells End1/E6E7:50% cell death at 12.5 µM.

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Amidation

• • Nonterminal Modifications and Unusual Amino Acids

  • Free

• • Stereochemistry

  • L

• • Structure

  • Not found

• • Structure Description

  • Not found

• • PDB ID

• Predicted Structure

• There is no predicted structure for DRAMP01555.

DRAMP01555

• • Function

  • Antibacterial peptide, that adopts an alpha helical conformation which can disrupt bacterial membranes. Each caerin displays a different antimicrobial specificity.

• • Tissue specificity

  • Expressed by the skin parotoid and/or rostral glands.

• • Domain

  • Contains two amphipathic alpha helix regions separated by a region of less-defined helicity and greater flexibility (By similarity).

• • PTM

  • C-terminal amidation.

• ·Literature 1

• • Title

  • Inhibition of HIV infection by caerin 1 antimicrobial peptides.

• • Pubmed ID

  • 26026377

• • Reference

  • Peptides. 2015 Sep;71:296-303.

• • Author

  • VanCompernolle S, Smith PB, Bowie JH, Tyler MJ, Unutmaz D, Rollins-Smith LA.

• ·Literature 2

• • Title

  • Host-defence peptides of Australian anurans: structure, mechanism of action and evolutionary significance.

• • Pubmed ID

  • 15203252

• • Reference

  • Peptides. 2004 Jun;25(6):1035-1054.

• • Author

  • Apponyi MA, Pukala TL, Brinkworth CS, Maselli VM, Bowie JH, Tyler MJ, Booker GW, Wallace JC, Carver JA, Separovic F, Doyle J, Llewellyn LE.

• ·Literature 3

• • Title

  • Peptides from Australian frogs. The structures of the caerins from Litoria caerula.

• • Pubmed ID

  • PubMed ID is not available

• • Reference

  • J. Chem. Res. 1993; 138: 910-936.

• • Author

  • Stone DJM, Waugh RJ, Bowie JH, Wallace JC, Tyler MJ.