General Information

    • DRAMP ID

    • DRAMP01563

    • Peptide Name

    • Caerin-2.2 (Frogs, amphibians, animals)

    • Source

    • Litoria gilleni (Centralian tree frog) (also Litoria caerulea) (Green tree frog)

    • Family

    • Belongs to the frog skin active peptide family (Caerin subfamily)

    • Gene

    • Not found

    • Sequence

    • GLVSSIGRALGGLLADVVKSKEQPA

    • Sequence Length

    • 25

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-positive bacterium: Micrococcus luteus (MIC=50 µg/ml);
      • Gram-negative bacterium: Pasteurella multocida (MIC=25 µg/ml).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01563 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP01563.

Physicochemical Information

    • Formula

    • C108H189N31O34

    • Absent Amino Acids

    • CFHMNTWY

    • Common Amino Acids

    • GL

    • Mass

    • 2465.88

    • PI

    • 8.59

    • Basic Residues

    • 3

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +1

    • Boman Index

    • -11.38

    • Hydrophobicity

    • 0.372

    • Aliphatic Index

    • 124.8

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 7

DRAMP01563

DRAMP01563 chydropathy plot

Comments Information

    • Function

    • Antimicrobial peptide, that adopts an alpha helical conformation which can disrupt bacterial membranes. Each caerin displays a different antimicrobial specificity.

    • Tissue specificity

    • Expressed by the skin parotoid and/or rostral glands.

Literature Information

  • ·Literature 1

    • Title

    • Host-defence peptides of Australian anurans: structure, mechanism of action and evolutionary significance.

    • Reference

    • Peptides. 2004 Jun;25(6):1035-1054.

    • Author

    • Apponyi MA, Pukala TL, Brinkworth CS, Maselli VM, Bowie JH, Tyler MJ, Booker GW, Wallace JC, Carver JA, Separovic F, Doyle J, Llewellyn LE.
  • ·Literature 2

    • Title

    • Peptides from Australian frogs. The structures of the caerins from Litoria caerula.

    • Reference

    • J. Chem. Res. 1993; 138: 910-936.

    • Author

    • Stone DJM, Waugh RJ, Bowie JH, Wallace JC, Tyler MJ.