• • DRAMP ID

  • DRAMP01577

• • Peptide Name

  • Caerin-1.10 (Frogs, amphibians, animals)

• • Source

  • Litoria rothii (Roth's tree frog); also Litoria splendida (Magnificent tree frog)

• • Family

  • Belongs to the frog skin active peptide family (Caerin subfamily)

• • Gene

  • Not found

• • Sequence

  • GLLSVLGSVAKHVLPHVVPVIAEKL

• • Sequence Length

  • 25

• • UniProt Entry

  • P86502,P82104

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-,Antiviral

• • Target Organism

  • • [Ref.10601876]Gram-positive bacteria: Leuconostoc lactis (MIC=6 µg/ml), Listeria innocua (MIC=50 µg/ml), Micrococcus luteus (MIC=25 µg/ml), Streptococcus uberis (MIC=50 µg/ml), Staphylococcus epidermidis (MIC=100 µg/ml);
    • Gram-negative bacterium: Pasteurella multocida (MIC=100 µg/ml).
    • [Ref.26026377]Virus:HIV: inhibition of HIV Pseudovirus (PsV) infection in CD4+ T cells(IC50=2.5 µM).

• • Hemolytic Activity

  • • No hemolysis information or data found in the reference(s) presented in this entry

• • Cytotoxicity

  • • [Ref.26026377]CD4+ T Lymphocytes:50% cell death at 22 µM.

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Amidation

• • Nonterminal Modifications and Unusual Amino Acids

  • Free

• • Stereochemistry

  • L

• • Structure

  • Not found

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP01577.

DRAMP01577

• • Function

  • Antibacterial peptide with wide spectrum of activity.

• • Tissue specificity

  • Expressed by the skin glands.

• • PTM

  • C-terminal amidation.

• ·Literature 1

• • Title

  • Inhibition of HIV infection by caerin 1 antimicrobial peptides.

• • Pubmed ID

  • 26026377

• • Reference

  • Peptides. 2015 Sep;71:296-303.

• • Author

  • VanCompernolle S, Smith PB, Bowie JH, Tyler MJ, Unutmaz D, Rollins-Smith LA.

• ·Literature 2

• • Title

  • The rothein peptides from the skin secretion of Roth's tree frog Litoria rothii. Sequence determination using positive and negative ion electrospray mass spectrometry.

• • Pubmed ID

  • 16124032

• • Reference

  • Rapid Commun Mass Spectrom. 2005;19(18):2716-2724.

• • Author

  • Brinkworth CS, Bowie JH, Bilusich D, Tyler MJ.

• ·Literature 3

• • Title

  • Differences in the skin peptides of the male and female Australian tree frog Litoria splendida. The discovery of the aquatic male sex pheromone splendipherin, together with phe8 caerulein and a new antibiotic peptide caerin 1.10.

• • Pubmed ID

  • 10601876

• • Reference

  • Eur J Biochem. 2000 Jan;267(1):269-275.

• • Author

  • Wabnitz PA, Bowie JH, Tyler MJ, Wallace JC, Smith BP.