General Information

    • DRAMP ID

    • DRAMP01618

    • Peptide Name

    • Aurein-3.3 (Frogs, amphibians, animals)

    • Source

    • Litoria raniformis (blue-thighed treefrog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GLFDIVKKIAGHIVSSI

    • Sequence Length

    • 17

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Anti-cancer

    • Target Organism

      • Gram-positive bacteria: Leuconostoc lactis (MIC=12 μg/ml), Micrococcus luteus (MIC=100 μg/ml), Staphylococcus epidermidis (MIC=50 μg/ml), Streptococcus uberis (MIC=25 μg/ml), Staphylococcus aureus (MIC=100 μg/ml);
      • Gram-negative bacterium: Pasteurella multocida (MIC=100 μg/ml)

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Cell membrane

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01618 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP01618.

Physicochemical Information

    • Formula

    • C84H141N21O22

    • Absent Amino Acids

    • CEMNPQRTWY

    • Common Amino Acids

    • I

    • Mass

    • 1797.17

    • PI

    • 8.6

    • Basic Residues

    • 3

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +2

    • Boman Index

    • 8.07

    • Hydrophobicity

    • 1.053

    • Aliphatic Index

    • 154.71

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 4

DRAMP01618

Comments Information

    • Function

    • The aurein antibiotics show more activity towards gram-positive than gram-negative pathogens. Probably acts by disturbing membrane functions with its amphipathic structure. Shows anticancer activity.

    • Tissue specificity

    • Expressed by the skin dorsal glands.

Literature Information

  • ·Literature 1

    • Title

    • The antibiotic and anticancer active aurein peptides from the Australian Bell Frogs Litoria aurea and Litoria raniformis the solution structure of aurein 1.2.

    • Reference

    • Eur J Biochem. 2000 Sep;267(17):5330-5341.

    • Author

    • Rozek T, Wegener KL, Bowie JH, Olver IN, Carver JA, Wallace JC, Tyler MJ.
  • ·Literature 2

    • Title

    • Host-defence peptides of Australian anurans: structure, mechanism of action and evolutionary significance.

    • Reference

    • Peptides. 2004 Jun;25(6):1035-1054.

    • Author

    • Apponyi MA, Pukala TL, Brinkworth CS, Maselli VM, Bowie JH, Tyler MJ, Booker GW, Wallace JC, Carver JA, Separovic F, Doyle J, Llewellyn LE.