General Information

    • DRAMP ID

    • DRAMP01651

    • Peptide Name

    • Dermaseptin-B4 (Dermaseptin BIV; Frogs, amphibians, animals)

    • Source

    • Phyllomedusa bicolor (Two-colored leaf frog) (Rana bicolor)

    • Family

    • Belongs to the frog skin active peptide family (Dermaseptin subfamily)

    • Gene

    • Not found

    • Sequence

    • ALWKDILKNVGKAAGKAVLNTVTDMVNQ

    • Sequence Length

    • 28

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-positive bacteria: Staphylococcus aureus ATCC 25293 (MIC=1.3 µM), S. aureus ATCC 25923 (MIC=3.0 µM);
      • Gram-negative bacteria: Pseudomonas aeruginosa CIP A22 (MIC=4.6 µM), Pseudomonas aeruginosa ATCC 27853 (MIC=11.6 µM), Escherichia coli ATCC 25922 (MIC=5.0 µM), E. coli 54127 (MIC=2.3 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01651 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C133H225N37O39S

    • Absent Amino Acids

    • CEFHPRSY

    • Common Amino Acids

    • AKV

    • Mass

    • 2998.53

    • PI

    • 9.53

    • Basic Residues

    • 4

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +2

    • Boman Index

    • -20.6

    • Hydrophobicity

    • 0.075

    • Aliphatic Index

    • 111.43

    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 203.7

    • Polar Residues

    • 7

DRAMP01651

DRAMP01651 chydropathy plot

Comments Information

    • Function

    • Possesses a potent antimicrobial activity against Gram-positive and Gram-negative bacteria. Probably acts by disturbing membrane functions with its amphipathic structure.

    • Tissue specificity

    • Expressed by the skin glands.

    • PTM

    • C-terminal amidation.

Literature Information

  • ·Literature 1

    • Title

    • Structure, synthesis, and molecular cloning of dermaseptins B, a family of skin peptide antibiotics.

    • Reference

    • J Biol Chem. 1998 Jun 12;273(24):14690-14697.

    • Author

    • Charpentier S, Amiche M, Mester J, Vouille V, Le Caer JP, Nicolas P, Delfour A.