General Information

    • DRAMP ID

    • DRAMP01697

    • Peptide Name

    • Dermaseptin-DA4 (DRS-DA4; Frogs, amphibians, animals)

    • Source

    • Pachymedusa dacnicolor (Mexican frog)

    • Family

    • Belongs to the frog skin active peptide family (Dermaseptin subfamily)

    • Gene

    • Not found

    • Sequence

    • GMWSKIKNAGKAAKAAAKAAGKAALGAVSEAM

    • Sequence Length

    • 32

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-

    • Target Organism

      • [Ref.FEBS J. 2009 Nov;276(22):6773-6786.]Gram-negative bacteria:Escherichia coli ML35p(MIC=5 µM), Escherichia coli 363 ATCC 11775(MIC=5 µM), Peudomonas aeruginosa ATCC 27853(MIC=40 µM).

    • Hemolytic Activity

      • [Ref. FEBS J. 2009 Nov;276(22):6773-6786.]Show no hemolytic activity against rat blood cells.

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01697 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C133H228N40O38S2

    • Absent Amino Acids

    • CDFHPQRTY

    • Common Amino Acids

    • A

    • Mass

    • 3059.64

    • PI

    • 10.3

    • Basic Residues

    • 6

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 16

    • Net Charge

    • +5

    • Boman Index

    • -7.16

    • Hydrophobicity

    • 0.106

    • Aliphatic Index

    • 70.94

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 177.42

    • Polar Residues

    • 7

DRAMP01697

DRAMP01697 chydropathy plot

Comments Information

    • It shows selective activity against gram-negative bacteria and not toxic to fresh rat erythrocytes. DRS-DA4 is a potent chemoattractant for human leukocytes and is devoid of hemolytic activity.

Literature Information

  • ·Literature 1

    • Title

    • Dermaseptin DA4, although closely related to dermaseptin B2, presents chemotactic and Gram-negative selective bactericidal activities.

    • Reference

    • FEBS J. 2009 Nov;276(22):6773-6786.

    • Author

    • Auvynet C, Joanne P, Bourdais J, Nicolas P, Lacombe C, Rosenstein Y.