General Information

    • DRAMP ID

    • DRAMP01730

    • Peptide Name

    • Temporin-A (Frogs, amphibians, animals)

    • Source

    • Rana temporaria (European common frog)

    • Family

    • Belongs to the frog skin active peptide (FSAP) family. Brevinin subfamily.

    • Gene

    • Not found

    • Sequence

    • FLPLIGRVLSGIL

    • Sequence Length

    • 13

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.9022710] Gram-positive bacteria: Bacillus megaterium BM11 (LC=1.2 µM), Staphylococcus aureus Cowan 1 (LC=2.3 µM), Streptococcus pyogenes betahemolytic group A (LC=2.0 µM);
      • Gram-negative bacteria: Escherichia coli D21 (LC=11.9 µM), Escherichia coli D21e7 (LC=1.4 µM), Escherichia coli D21f1 (LC=0.9 µM), Escherichia coli D21f2 (LC=4.8 µM), Escherichia coli D22 (LC=3.4 µM), Yersinia pseudotuberculosis (LC=2.0 µM), Pseudomonas aeruginosa ATCC 15692 (LC>360 µM);
      • Yeast: Candida albicans ATCC 10261 (LC=3.4 µM).(LC: Lethal concentration)

    • Hemolytic Activity

      • [Ref.9022710] LC>120 µM against Human red blood cells. (LC: Lethal concentration)

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01730 helical wheel diagram

    • PDB ID

    • 2MAA resolved by NMR.

    • Predicted Structure

    • There is no predicted structure for DRAMP01730.

Physicochemical Information

    • Formula

    • C68H116N16O15

    • Absent Amino Acids

    • ACDEHKMNQTWY

    • Common Amino Acids

    • L

    • Mass

    • 1397.77

    • PI

    • 9.75

    • Basic Residues

    • 1

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +1

    • Boman Index

    • 20.1

    • Hydrophobicity

    • 1.808

    • Aliphatic Index

    • 202.31

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP01730

Comments Information

    • Function

    • Antimicrobial activity against Gram-positive, Gram-negative bacteria and fungi. Has hemolytic activity.

    • PTM

    • Leucine amide at position 13.

    • Tissue specificity

    • Expressed by the skin glands.

Literature Information

  • ·Literature 1

    • Title

    • Temporins, antimicrobial peptides from the European red frog Rana temporaria.

    • Reference

    • Eur J Biochem. 1996 Dec 15;242(3):788-792.

    • Author

    • Simmaco M, Mignogna G, Canofeni S, Miele R, Mangoni ML, Barra D.