General Information

    • DRAMP ID

    • DRAMP01739

    • Peptide Name

    • Temporin-B (Frogs, amphibians, animals)

    • Source

    • Rana temporaria (European common frog)

    • Family

    • Belongs to the frog skin active peptide (FSAP) family. Brevinin subfamily.

    • Gene

    • Not found

    • Sequence

    • LLPIVGNLLKSLL

    • Sequence Length

    • 13

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.9022710] Gram-positive bacteria: Bacillus megaterium BM11 (LC=2.8 µM), Staphylococcus aureus Cowanl (LC=6.0 µM), Streptococcus pyogenes betahemolytic group A (LC=7.0 µM);
      • Gram-negative bacteria: Escherichia coli D21(LC=21.0 µM), Escherichia coli D21e7 (LC=13.2 µM), Escherichia coli D21f1 (LC=10.0 µM), Escherichia coli D21f2 (LC=3.3 µM), Escherichia coli D22 (LC=11.2 µM),Yersinia pseudotuberculosis (LC=7.0 µM), Pseudomonas aeruginosa ATCC15692 (LC>360 µM);
      • Yeast: Candida albicans (LC=4.0 µM).(LC: Lethal concentration).
      • [Ref.28443279] Gram-positive bacterium: S.aureus ATCC 33591 (MIC=12μM), Staphylococcus epidermidis (MIC=6μM);
      • Gram-negative bacterium: Klebsiella pneumoniae ATCC BAA-1706 (MIC=48μM), Pseudomonas aeruginosa ATCC 27853 (MIC=48μM)

    • Hemolytic Activity

      • [Ref.9022710] LC>120 µM against Human red blood cells. (LC: Lethal concentration)
      • [Ref.28443279] 1% hemolysis at 96μM against human red blood cells.

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01739 helical wheel diagram

    • PDB ID

    • 6GIL resolved by NMR.

    • Predicted Structure

    • There is no predicted structure for DRAMP01739.

Physicochemical Information

    • Formula

    • C67H121N15O16

    • Absent Amino Acids

    • ACDEFHMQRTWY

    • Common Amino Acids

    • L

    • Mass

    • 1392.79

    • PI

    • 8.75

    • Basic Residues

    • 1

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +1

    • Boman Index

    • 23.83

    • Hydrophobicity

    • 1.639

    • Aliphatic Index

    • 232.31

    • Half Life

      • Mammalian:5.5 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP01739

Comments Information

    • Function

    • Antimicrobial activity against Gram-positive, Gram-negative bacteria and fungi. Has hemolytic activity.

    • PTM

    • Leucine amide at position 13.

    • Tissue specificity

    • Expressed by the skin glands.

Literature Information

  • ·Literature 1

    • Title

    • Temporins, antimicrobial peptides from the European red frog Rana temporaria.

    • Reference

    • Eur J Biochem. 1996 Dec 15;242(3):788-792.

    • Author

    • Simmaco M, Mignogna G, Canofeni S, Miele R, Mangoni ML, Barra D.
  • ·Literature 2

    • Title

    • Analogs of the Frog-skin Antimicrobial Peptide Temporin 1Tb Exhibit a Wider Spectrum of Activity and a Stronger Antibiofilm Potential as Compared to the Parental Peptide

    • Reference

    • Front Chem. 2017 Apr 11;5:24.

    • Author

    • Grassi L, Maisetta G, Maccari G, Esin S, Batoni G.