General Information

    • DRAMP ID

    • DRAMP01753

    • Peptide Name

    • Temporin-1CEa (Frogs, amphibians, animals)

    • Source

    • Rana chensinensis (Chinese brown frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • FVDLKKIANIINSIFGK

    • Sequence Length

    • 17

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.21871946]Gram-positive bacteria: Staphylococcus aureus (MIC=14.4 µM), Bacillus cereus (MIC=14.4 µM), Streptococcus lactis (MIC=14.4 µM);
      • Gram-negative bacterium: Escherichia coli (MIC>100 µM).

    • Hemolytic Activity

      • [Ref:21871946]LD50=99.08 μM against human erythrocytes

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01753 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP01753.

Physicochemical Information

    • Formula

    • C91H150N22O23

    • Absent Amino Acids

    • CEHMPQRTWY

    • Common Amino Acids

    • I

    • Mass

    • 1920.33

    • PI

    • 9.7

    • Basic Residues

    • 3

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +2

    • Boman Index

    • -4.7

    • Hydrophobicity

    • 0.588

    • Aliphatic Index

    • 137.65

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 4

DRAMP01753

Comments Information

    • Function

    • Temporin-1CEa exhibits the greatest ability to inhibit the growth of MCF-7 cells. Also temporin-1CEa shows hemolytic effect to human erythrocytes and has no significant cytotoxicity to normal HUVSMCs at concentrations showed potent antitumor activity.

Literature Information

  • ·Literature 1

    • Title

    • Antitumor effects and cell selectivity of temporin-1CEa, an antimicrobial peptide from the skin secretions of the Chinese brown frog (Rana chensinensis).

    • Reference

    • Biochimie. 2012 Feb;94(2):434-441.

    • Author

    • Wang C, Li HB, Li S, Tian LL, Shang DJ.
  • ·Literature 2

    • Title

    • Molecular cloning of cDNAs encoding antimicrobial peptide precursors from the skin of the Chinese brown frog, Rana chensinensis.

    • Reference

    • Zoolog Sci. 2009 Mar;26(3):220-226.

    • Author

    • Shang D, Yu F, Li J, Zheng J, Zhang L, Li Y.