General Information
-
DRAMP ID
- DRAMP01775
-
Peptide Name
- Temporin-1Sa (Frogs, amphibians, animals)
-
Source
- Pelophylax saharicus (Sahara frog) (Rana saharica)
-
Family
- Belongs to the frog skin active peptide family (Brevinin subfamily)
-
Gene
- preproTemp-1Sa
-
Sequence
- FLSGIVGMLGKLFGK
-
Sequence Length
- 15
-
UniProt Entry
- B3KYH4
-
Protein Existence
- Transcript level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
-
Target Organism
-
- [Ref.18584916]Gram-positive bacteria: Staphylococcus aureus ATCC 25923 (MIC=3 µM), Enterococcus faecalis ATCC 29212 (MIC=10 µM), Bacillus megaterium (MIC=2 µM);
- Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC=10 µM), Escherichia coli ATCC 35218 (MIC=10 µM), Pseudomonas aeruginosa ATCC 27853 (MIC=31 µM);
- Fungi: Candida albicans ATCC 90028 (MIC=16 µM), Candida parapsilosis ATCC 22019 (MIC=31 µM), Saccharomyces cerevisiae (MIC=8 µM).
-
Hemolytic Activity
-
- [Ref:18584916]LC50=25 μM against human erythrocytes
-
Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- Free
-
Stereochemistry
- L
-
Structure
- Not found
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP01775.
Physicochemical Information
-
Formula
- C75H123N17O17S
Absent Amino Acids
- ACDEHNPQRTWY
Common Amino Acids
- G
Mass
- 1566.97
PI
- 10
Basic Residues
- 2
Acidic Residues
- 0
Hydrophobic Residues
- 7
Net Charge
- +2
-
Boman Index
- 21.29
Hydrophobicity
- 1.16
Aliphatic Index
- 123.33
Half Life
-
- Mammalian:1.1 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 5
DRAMP01775
Comments Information
Function
- Activity against Gram-positive and Gram-negative bacteria, and has hemolytic activity against human erythrocytes.
Literature Information
- ·Literature 1
-
Title
- Isolation, characterization and molecular cloning of new temporins from the skin of the North African ranid Pelophylax saharica.
-
Pubmed ID
- 18584916
-
Reference
- Peptides. 2008 Sep;29(9):1526-1523.
-
Author
- Abbassi F, Oury B, Blasco T, Sereno D, Bolbach G, Nicolas P, Hani K, Amiche M, Ladram A