General Information

    • DRAMP ID

    • DRAMP01777

    • Peptide Name

    • Temporin-1Sc (Temporin-SHc; Frogs, amphibians, animals)

    • Source

    • Pelophylax saharicus (Sahara frog) (Rana saharica)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • preproTemp-1Sc

    • Sequence

    • FLSHIAGFLSNLFGK

    • Sequence Length

    • 15

    • Protein Existence

    • Transcript level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.18584916]Gram-positive bacteria: Staphylococcus aureus ATCC 25923 (MIC=10 µg/ml), Enterococcus faecalis ATCC 29212 (MIC>80 µg/ml), Bacillus megaterium (MIC=4 µg/ml);
      • Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC=161 µg/ml), Escherichia coli ATCC 35218 (MIC>80 µg/ml), Pseudomonas aeruginosa ATCC 27853 (MIC>161 µg/ml);
      • Fungi: Aspergillus flavus (MIC=10 µg/ml), Candida albicans ATCC 90028 (MIC=20 µg/ml), Candida parapsilosis ATCC 22019 (MIC=20 µg/ml), Saccharomyces cerevisiae (MIC=10 µg/ml).

    • Hemolytic Activity

      • [Ref:18584916]LC50>80 μM against human erythrocytes

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01777 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP01777.

Physicochemical Information

    • Formula

    • C80H119N19O19

    • Absent Amino Acids

    • CDEMPQRTVWY

    • Common Amino Acids

    • FL

    • Mass

    • 1650.94

    • PI

    • 8.76

    • Basic Residues

    • 2

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +2

    • Boman Index

    • 8.66

    • Hydrophobicity

    • 0.873

    • Aliphatic Index

    • 110.67

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 5

DRAMP01777

Comments Information

    • Function

    • Activity against a range of Gram-positive and Gram-negative bacteria, and has hemolytic activity against human erythrocytes.

Literature Information

  • ·Literature 1

    • Title

    • Isolation, characterization and molecular cloning of new temporins from the skin of the North African ranid Pelophylax saharica.

    • Reference

    • Peptides. 2008 Sep;29(9):1526-1523.

    • Author

    • Abbassi F, Oury B, Blasco T, Sereno D, Bolbach G, Nicolas P, Hani K, Amiche M, Ladram A