General Information

    • DRAMP ID

    • DRAMP01812

    • Peptide Name

    • Temporin-Rb (Frogs, amphibians, animals)

    • Source

    • Rana ridibunda (marsh frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • FLPVLAGVLSRA

    • Sequence Length

    • 12

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-positive bacteria: Streptococcus dysgalactiae PTCC 1074 (MIC=21.8 µg/ml), Streptococcus agalactiae PTCC 1365 (MIC=30.5 µg/ml), Staphylococcus aureus PTCC 1431 (MIC=42.1 µg/ml), Staphylococcus typhimurium (MIC=31.8 µg/ml);
      • Gram-negative bacteria: Escherichia coli (MIC=34.5 µg/ml), Klebsiella pneumoniae PTCC 1388 (MIC=35.1 µg/ml).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01812 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C59H99N15O14

    • Absent Amino Acids

    • CDEHIKMNQTWY

    • Common Amino Acids

    • L

    • Mass

    • 1242.53

    • PI

    • 9.75

    • Basic Residues

    • 1

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +1

    • Boman Index

    • 12.06

    • Hydrophobicity

    • 1.575

    • Aliphatic Index

    • 162.5

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 2

DRAMP01812

Comments Information

    • At 60 ug/ml, the peptide showed about 1.1% hemolysis.

Literature Information

  • ·Literature 1

    • Title

    • Identification and characterization of two novel antimicrobial peptides, temporin-Ra and temporin-Rb, from skin secretions of the marsh frog (Rana ridibunda).

    • Reference

    • J Pept Sci. 2012 Jan;18(1):10-16.

    • Author

    • Asoodeh A, Zardini HZ, Chamani J.