General Information

    • DRAMP ID

    • DRAMP01849

    • Peptide Name

    • Jindongenin-1a (JD1a; Frogs, amphibians, animals)

    • Source

    • Amolops jingdongensis (Chinese torrent frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • DSMGAVKLAKLLIDKMKCEVTKAC

    • Sequence Length

    • 24

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.21816202]Gram-positive bacteria: Staphylococcus aureus ATCC25923 (MIC=17 µM), Enterococcus faecalis ATCC29212 (MIC=60 µM), Staphylococcus aureus (IS 10#)(MIC=40 µM);
      • Gram-negative bacteria: Escherichia coli ATCC25922 (MIC=10 µM), Enterobacter cloacae ATCC13047 (MIC=40 µM), Klebsiella pneumoniae ATCC700603 (MIC=15 µM), Bacillus pyocyaneus CMCCB1010 (MIC=17 µM), Pseudomonas aeruginosa ATCC27853 (MIC=23 µM), Bacillus dysenteriae (MIC=40 µM);
      • Yeast: Candida albicans ATCC2002 (MIC=60 µM).

    • Hemolytic Activity

      • [Ref.21816202] IC50=150 μM against washed human erythrocytes

    • Cytotoxicity

      • [Ref.21816202] Cytotoxicity: the K562 (IC50=46μM)and HT29 mammalian cell lines(IC50=40μM).

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys18 and Cys24)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys18 and Cys24.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01849 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C111H199N29O33S4

    • Absent Amino Acids

    • FHNPQRWY

    • Common Amino Acids

    • K

    • Mass

    • 2596.22

    • PI

    • 8.79

    • Basic Residues

    • 5

    • Acidic Residues

    • 3

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +2

    • Boman Index

    • -16.58

    • Hydrophobicity

    • 0.275

    • Aliphatic Index

    • 101.67

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 5.43

    • Polar Residues

    • 5

DRAMP01849

DRAMP01849 chydropathy plot

Comments Information

    • Function

    • Displays broad-spectrum antibacterial activity against a range of Gram-positive and Gram-negative bacteria. Also displays antifungal activity against C. albicans ATCC 2002. Has hemolytic activity, low cytotoxicity and low antioxidant activity.

Literature Information

  • ·Literature 1

    • Title

    • Two novel families of antimicrobial peptides from skin secretions of the Chinese torrent frog, Amolops jingdongensis.

    • Reference

    • Biochimie. 2012 Feb;94(2):328-334.

    • Author

    • Chen Z, Yang X, Liu Z, Zeng L, Lee W, Zhang Y.