General Information

    • DRAMP ID

    • DRAMP01873

    • Peptide Name

    • Brevinin-2-related peptide (Frogs, amphibians, animals)

    • Source

    • Rana septentrionalis (mink frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GIWDTIKSMGKVFAGKILQNL

    • Sequence Length

    • 21

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.15556063]Gram-negative bacterium: Escherichia coli (MIC=13 µM);
      • Gram-positive bacterium: Staphylococcus aureus (MIC=25 µM);
      • Yeast: Candida albicans (MIC=70 µM).

    • Hemolytic Activity

      • [Ref.15556063]HC50=70 µM against human erythrocytes

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01873 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C107H175N27O28S

    • Absent Amino Acids

    • CEHPRY

    • Common Amino Acids

    • GIK

    • Mass

    • 2319.79

    • PI

    • 9.7

    • Basic Residues

    • 3

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +2

    • Boman Index

    • -2.59

    • Hydrophobicity

    • 0.286

    • Aliphatic Index

    • 111.43

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 275

    • Polar Residues

    • 6

DRAMP01873

DRAMP01873 chydropathy plot

Comments Information

    • Function

    • Has inhibitory activity towards reference strains of bacteria and an opportunistic yeast pathogen. Also has hemolytic activity against human erythrocytes.

    • Sequence similarity

    • This peptide shows limited structural similarity with the N-terminal region of brevinin-2 peptides previously isolated from R. temporaria skin but lacks the C-terminal cyclic heptapeptide domain associated with this family.

    • PTM

    • C-terminal amidation.

Literature Information

  • ·Literature 1

    • Title

    • Purification and characterization of antimicrobial peptides from the skin secretions of the mink frog (Rana septentrionalis).

    • Reference

    • Comp Biochem Physiol C Toxicol Pharmacol. 2004 Oct;139(1-3):31-38.

    • Author

    • Bevier CR, Sonnevend A, Kolodziejek J, Nowotny N, Nielsen PF, Conlon JM.