General Information

    • DRAMP ID

    • DRAMP01891

    • Peptide Name

    • Brevinin-1VLc (Frogs, amphibians, animals)

    • Source

    • Rana vaillanti (Central American frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • FLPVIASVAAKVLPKVFCFITKKC

    • Sequence Length

    • 24

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-negative bacterium: E. coli (MIC=50 µM);
      • Gram-positive bacterium: S. aureus (MIC=1.5 µM).
      • Yeast: C. albicans (MIC=3 µM).

    • Hemolytic Activity

      • [Ref.19379837] LC50=7 mM against human erythrocytes

    • Cytotoxicity

      • [Ref.19379837] LC50=4mM against HepG2 hepatoma-derived cells

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys18 and Cys24)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys18 and Cys24.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01891 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C127H208N28O27S2

    • Absent Amino Acids

    • DEGHMNQRWY

    • Common Amino Acids

    • KV

    • Mass

    • 2623.34

    • PI

    • 9.7

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 14

    • Net Charge

    • +4

    • Boman Index

    • 24.6

    • Hydrophobicity

    • 1.329

    • Aliphatic Index

    • 125.83

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 5.43

    • Polar Residues

    • 4

DRAMP01891

DRAMP01891 chydropathy plot

Comments Information

    • Function

    • Brevinin-1VLc showes particularly high growth-inhibitory potency (MIC < or =3 microM) against a Gram-positive microorganism Staphylococcus aureus and the opportunistic yeast pathogen Candida albicans and potent cytolytic activity (LC(50)< or =8 microM) against both human erythrocytes and HepG2 hepatoma-derived cells. The peptide ia also active against a Gram-negative microorganism Escherichia coli (MIC< or =50 microM).

Literature Information

  • ·Literature 1

    • Title

    • Purification of peptides with differential cytolytic activities from the skin secretions of the Central American frog, Lithobates vaillanti (Ranidae).

    • Reference

    • Comp Biochem Physiol C Toxicol Pharmacol. 2009 Aug;150(2):150-154.

    • Author

    • Conlon JM, Raza H, Coquet L, Jouenne T, Leprince J, Vaudry H, King JD.