General Information
-
DRAMP ID
- DRAMP01910
-
Peptide Name
- Brevinin-2GHb (AMP-2; Frogs, amphibians, animals)
-
Source
- Rana guentheri (Gunther's frog)
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Family
- Belongs to the frog skin active peptide family (Brevinin subfamily)
-
Gene
- br2GHb
-
Sequence
- GVITDALKGAAKTVAAELLRKAHCKLTNSC
-
Sequence Length
- 30
-
UniProt Entry
- A0AEI5
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Target Organism
-
- Gram-positive bacteria: Staphylococcus aureus FDA209P (MIC=16.5 µg/ml), Bacillus subtilis ATCC 6633 (MIC>64 µg/ml);
- Gram-negative bacteria: Escherichia coli O111 (MIC=8.2 µg/ml), Escherichia coli ATCC 25922 (MIC=8.2 µg/ml).
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Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- Free
-
C-terminal Modification
- Cyclization (Cys24 and Cys30)
-
Nonterminal Modifications and Unusual Amino Acids
- Disulfide bond between Cys24 and Cys30.
-
Stereochemistry
- L
-
Structure
- Not found
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP01910.
Physicochemical Information
-
Formula
- C132H232N40O40S2
Absent Amino Acids
- FMPQWY
Common Amino Acids
- A
Mass
- 3083.66
PI
- 9.31
Basic Residues
- 6
Acidic Residues
- 2
Hydrophobic Residues
- 13
Net Charge
- +4
-
Boman Index
- -27.08
Hydrophobicity
- 0.213
Aliphatic Index
- 104.33
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 125
Absorbance 280nm
- 4.31
Polar Residues
- 9
DRAMP01910
Comments Information
Function
- Antimicrobial peptide.
Tissue specificity
- Expressed by the skin glands.
PTM
- Problely contains one disulfide bond 24-30.
Literature Information
- ·Literature 1
-
Title
- Purification and characterization of novel antimicrobial peptides from the skin secretion of Hylarana guentheri.
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Pubmed ID
- 16979798
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Reference
- Peptides. 2006 Dec;27(12):3077-3084.
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Author
- Zhou J, McClean S, Thompson A, Zhang Y, Shaw C, Rao P, Bjourson AJ.