General Information

    • DRAMP ID

    • DRAMP01914

    • Peptide Name

    • Brevinin-2GRa (Frogs, amphibians, animals)

    • Source

    • Rana grahami (Asian frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • GLLDTFKNLALNAAKSAGVSVLNSLSCKLSKTC

    • Sequence Length

    • 33

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-negative bacterium: Escherichia coli (MIC=12.5 µM);
      • Gram-positive bacterium: Staphylococcus aureus (MIC=50 µM).
      • Yeast: Candida albicans (MIC>100 µM).

    • Hemolytic Activity

      • [Ref.16621155] LC50=140 μM against human erythrocytes.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys27 and Cys33)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys27 and Cys33.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01914 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP01914.

Physicochemical Information

    • Formula

    • C146H252N40O46S2

    • Absent Amino Acids

    • EHIMPQRWY

    • Common Amino Acids

    • L

    • Mass

    • 3367.97

    • PI

    • 9.31

    • Basic Residues

    • 4

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 14

    • Net Charge

    • +3

    • Boman Index

    • -15.8

    • Hydrophobicity

    • 0.43

    • Aliphatic Index

    • 112.42

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 3.91

    • Polar Residues

    • 14

DRAMP01914

DRAMP01914 chydropathy plot

Comments Information

    • Comment

    • No comments found on DRAMP database

Literature Information

  • ·Literature 1

    • Title

    • Antimicrobial peptides from diverse families isolated from the skin of the Asian frog, Rana grahami.

    • Reference

    • Peptides. 2006 Sep;27(9):2111-2117.

    • Author

    • Conlon JM, Al-Ghaferi N, Abraham B, Jiansheng H, Cosette P, Leprince J, Jouenne T, Vaudry H.
  • ·Literature 2

    • Title

    • Anti-infection peptidomics of amphibian skin.

    • Reference

    • Mol Cell Proteomics. 2007 May;6(5):882-894.

    • Author

    • Li J, Xu X, Xu C, Zhou W, Zhang K, Yu H, Zhang Y, Zheng Y, Rees HH, Lai R, Yang D, Wu J.